AbPlus™ Anti-VEGFA Magnetic Beads (A.4.6.1)

CAT#: VS-0424-XY273

The AbPlus Anti-VEGFA Magnetic Beads (A.4.6.1) is an innovative affinity resin which is bound with anti-VEGFA specific antibody (A.4.6.1). The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of VEGFA protein under native and denaturing conditions.

Gene Expression
Figure 1 Cerebral cortex Figure 2 Thyroid gland Figure 3 Colon Figure 4 Liver Figure 5 Kidney Figure 6 Testis Figure 7 Lymph node Figure 8 RNA cell line category: Cell line enhanced (hTERT-RPE1, U-87 MG) Figure 8 RNA cell line category: Cell line enhanced (hTERT-RPE1, U-87 MG)

Specifications

  • Applications
  • Immunoprecipitation, Protein Purification
  • Matrix
  • Magrose bead (> 50 μmol/mL gel)
  • Bead Ligand
  • Anti-VEGFA specific antibody (A.4.6.1)
  • Target
  • VEGFA
  • Immunogen
  • Recombinant human VEGFA
  • Target Species
  • Human, Rat
  • Antibody Clone
  • A.4.6.1
  • Bead Capacity
  • 20-30 mg/mL binding antibody
  • Bead size
  • 10-37 μm
  • Stability
  • pH 2-14
  • Format
  • 20% Suspension
  • Buffer
  • PBS, pH 7.4, with 1% BSA
  • Preservative
  • 0.03% Proclin 300
  • Storage
  • Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.

Applications

  • Application Notes
  • The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.

Target

  • Introduction
  • This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms.
  • Alternative Names
  • VEGFA; vascular endothelial growth factor A; VPF; VEGF; MVCD1; vascular permeability factor
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