Rabbit Anti-WNT2B Recombinant Antibody (clone R01-8F5)
CAT#: VS3-FY1939
This R01-8F5 recombinant monoclonal antibody is specifically created to target human WNT2B. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, ICC/IF. This wnt2b antibody has been rigorously validated and is widely used in western blot analysis of Wnt2b in K562, C6, 3T3, Hela lysates and immunocytochemistry analysis of Wnt2b (green) in 293t using Wnt2b antibody.
Tested Data
WB
Figure 1 Anti-WNT2B Antibody (VS3-FY1939) in WB.
The Wnt2b expression in K562, C6, 3T3, Hela lysates were detected using the anti-Wnt2b by western blot assay.
ICC
Figure 2 Anti-WNT2B Antibody (VS3-FY1939) in ICC.
Immunocytochemistry staining of Wnt2b (green) in 293t using the anti-Wnt2b antibody. Nuclear DNA was labeled with DAPI (shown in blue).
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Specifications
- Immunogen
- Synthetic peptide of human Wnt2b
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat WNT2B
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R01-8F5
- Applications
- Western Blot, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 44 kDa, while the observed molecular weight stands at 44 kDa.
- Related Disease
- Diarrhea 9 and Diarrhea
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Wnt Family Member 2B; Wingless-Type MMTV Integration Site Family, Member 13; Wingless-Type MMTV Integration Site Family, Member 2B; XWNT2, Xenopus, Homolog Of; WNT13; Protein Wnt-2b; Protein Wnt-13
- Gene ID
- 7482
- UniProt ID
- Q93097
- Sequence Similarities
- Belongs to the Wnt family.
- Cellular Localization
- Extracellular matrix, Secreted
- Post Translation Modifications
- Palmitoleoylation is required for efficient binding to frizzled receptors.
Depalmitoleoylation leads to Wnt signaling pathway inhibition.
(Q93097-WNT2B_HUMAN)
Glycosylation at Asn117 and Asn283 (NX_Q93097 [NX_Q93097-1])
Modification sites at PhosphoSitePlus (Q93097)
Glycosylation from GlyGen (Q93097) 2 sites
- Protein Refseq
- NP_001278809.1; NP_004176.2; NP_078613.1
- Function
- Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt/beta-catenin signaling pathway. Plays a redundant role in embryonic lung development.
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Related Signaling Pathways
Canonical Wnt Signaling Pathway
mTOR Signaling Pathway
Non-Canonical Wnt Signaling Pathway
Related Diseases
Endometrial Cancer
Throid Cancer
Cushing Syndrome
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "WNT2B"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3880 | Rabbit Anti-WNT2B Recombinant Antibody (clone DS3880AB) | ICC, IF, WB, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1599-CN | Rabbit Anti-WNT2B Recombinant Antibody (clone JB48-34) | WB, IHC, IF, FC | Rabbit IgG |
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