Mouse Anti-WT1 Recombinant Antibody (clone ABT-WT1)
CAT#: ZG-0176C
This product is a mouse antibody that recognizes WT1. It can be used in a wide variety of applications, such as: IHC-P, IF.
Tested Data
IHC
Figure 1 Mouse Anti-WT1 Antibody (clone ABT-WT1) in IHC
Immunohistochemical analysis of paraffin-embedded Mesothelioma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min).
IHC
Figure 2 Mouse Anti-WT1 Antibody (clone ABT-WT1) in IHC
Immunohistochemical analysis of paraffin-embedded Mesothelioma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min).
IHC
Figure 3 Mouse Anti-WT1 Antibody (clone ABT-WT1) in IHC
Immunohistochemical analysis of paraffin-embedded Ovarian serouscystadeno carcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min).
IHC
Figure 4 Mouse Anti-WT1 Antibody (clone ABT-WT1) in IHC
Immunohistochemical analysis of paraffin-embedded Wilms' tumor. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min).
IHC
Figure 5 Mouse Anti-WT1 Antibody (clone ABT-WT1) in IHC
Immunohistochemical analysis of paraffin-embedded Wilms' tumor. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min).
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Specifications
- Immunogen
- Synthesized peptide derived from human Wilms' Tumor 1(WT1)
- Host Species
- Mouse
- Type
- Mouse IgG
- Antibody Isotype
- IgG
- Species Reactivity
- Human, Mouse
- Clone
- ABT-WT1
- Applications
- IHC-P, IF
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- 1 mg/mL
- Storage
- Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Reliable Detection In Urogenital Tissues
I used this Mouse Anti-WT1 antibody for my research on the development of the urogenital system. The staining in IHC-P applications was incredibly distinct, allowing us to visualize the complex tissue-specific expression patterns of the transcription factor. The results were consistent across multiple tissue samples.
Excellent Specificity For Wilms Tumor
Our lab has been studying Wilms tumor variants, and this antibody (clone ABT-WT1) performed exceptionally well in our immunofluorescence assays. It accurately recognized the WT1 protein, and we observed minimal background signal. It has become a staple reagent for our ongoing characterization of zinc-finger motif proteins.
Q&As
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What are the recommended applications for this Anti-WT1 antibody?
A: This product is a mouse recombinant antibody that has been validated for use in Immunohistochemistry (Paraffin) and Immunofluorescence. It specifically recognizes the WT1 transcription factor and is suitable for research involving urogenital development and Wilms tumor.
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Does this antibody cross-react with species other than humans?
A: Yes, this antibody is known to react with both Human and Mouse WT1. It targets a synthesized peptide derived from human Wilms' Tumor 1, which shares homology with the mouse protein, making it versatile for comparative research in these species.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "WT1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1458z | Mouse Anti-WT1 Recombinant Antibody (clone 24C8) | FC, IF, IHC | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G065R | Anti-WT1 immunotoxin 6F-H2 (IgG)-RTA | Cytotoxicity assay, Function study |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0344MZ | Recombinant Mouse Anti-Human Wilms Tumor 1 Antibody (clone 7G-H3) | IHC | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC092 | A*24:02/Human Modified WT1 (CYTWNQMNL) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC093 | A*02:01/Human WT-1 (RMFPNAPYL) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC111 | PE-A*24:02/Human WT1 (mutant) (CYTWNQMNL) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC112 | APC-A*24:02/Human WT1 (mutant) (CYTWNQMNL) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC113 | PE-A*02:01/Human WT1 (RMFPNAPYL) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3886 | Rabbit Anti-WT1 Recombinant Antibody (clone DS3886AB) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-117CQ | Human Anti-WT1 Recombinant Antibody (MOB-117CQ) | ELISA, WB, FC, FuncS | Human IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1597-CN | Rabbit Anti-WT1 Recombinant Antibody (clone CBACN-584) | WB, IF, IHC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0177C | Mouse Anti-WT1 Recombinant Antibody (clone PT0264) | IHC-P, IF | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY545 | Mouse Anti-NHP WT1 Recombinant Antibody (clone 7F9E8) | WB, IHC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7945 | Anti-WT1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7946 | Anti-Mouse WT1 Immunohistochemistry Kit | IHC |
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