Mouse Anti-XRCC4 Recombinant Antibody (clone 5C10) (CAT#: ZG-0178C)
This product is a mouse antibody that recognizes XRCC4. It can be used in a wide variety of applications, such as: WB, IHC-P, IF, IP.
Specific Inquiry
IF
Figure 1 Mouse Anti-XRCC4 Antibody (clone 5C10) in IF
Immunofluorescence analysis of Hela cell. 1, Bak Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). XRCC4 monoclonal Antibody (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti-Rabbit Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50 min). Goat Anti-Mouse Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50 min).
IHC
Figure 2 Mouse Anti-XRCC4 Antibody (clone 5C10) in IHC
Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1, XRCC4 monoclonal antibody (5C10) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min). Negative control was used by secondary antibody only.
IF
Figure 3 Mouse Anti-XRCC4 Antibody (clone 5C10) in IF
Immunofluorescence analysis of Human-liver-cancer tissue. 1, XRCC4 monoclonal antibody (5C10) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50 min). 3, Picture B: DAPI (blue) 10 min. Picture A:Target. Picture B: DAPI.Picture C: merge of A+B
WB
Figure 4 Mouse Anti-XRCC4 Antibody (clone 5C10) in WB
Western blot analysis of 1) Hela, 2) 293T, diluted at 1:3000. 1) Hela Cell Lysate 2) IP product: IP dilute 1:200
IP
Figure 5 Mouse Anti-XRCC4 Antibody (clone 5C10) in IP
1) Hela Cell Lysate 2) IP product: IP dilute 1:200
Specifications
- Immunogen
- Synthetic Peptide of XRCC4
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human XRCC4
- Species Reactivity
- Human
- Clone
- 5C10
- Applications
- WB, IHC-P, IF, IP
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- 1 mg/mL
- Storage
- Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-WK933 | Mouse Anti-XRCC4 Recombinant Antibody (VS3-WK933) | WB, IHC, IP | Mouse IgG |
| VS3-FY2921 | Mouse Anti-XRCC4 Recombinant Antibody (VS3-FY2921) | WB, IHC, ICC, IP | Mouse IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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