XRCC4

Cancer-related genes, Disease related genes, Human disease related genes

Intracellular

Cell type enhanced (Oligodendrocytes, Pancreatic endocrine cells)

Low immune cell specificity

Cell line enhanced (RH-30)

[DNA repair protein XRCC4]: Homodimer and homotetramer in solution (PubMed:25574025, PubMed:25670504, PubMed:25941166, PubMed:31548606, PubMed:11080143). Interacts with NHEJ1/XLF; the interaction is direct and is mediated via a head-to-head interaction between N-terminal head regions (PubMed:16439205, PubMed:18158905, PubMed:20558749, PubMed:22228831, PubMed:26100018, PubMed:27437582, PubMed:21936820, PubMed:21775435, PubMed:22287571, PubMed:21768349). Interacts with LIG4; the LIG4-XRCC4 subcomplex has a 1:2 stoichiometry and XRCC4 is required for LIG4 stability (PubMed:9259561, PubMed:12517771, PubMed:17290226, PubMed:21982441, PubMed:24984242, PubMed:25934149, PubMed:11702069, PubMed:19332554, PubMed:9242410). Component of the core long-range non-homologous end joining (NHEJ) complex (also named DNA-PK complex) composed of PRKDC, LIG4, XRCC4, XRCC6/Ku70, XRCC5/Ku86 and NHEJ1/XLF (PubMed:10757784, PubMed:10854421, PubMed:17124166, PubMed:12547193, PubMed:26774286, PubMed:33854234). Additional component of the NHEJ complex includes PAXX (PubMed:16439205). Following autophosphorylation, PRKDC dissociates from DNA, leading to formation of the short-range NHEJ complex, composed of LIG4, XRCC4, XRCC6/Ku70, XRCC5/Ku86 and NHEJ1/XLF (PubMed:33854234). Interacts with PRKDC; the interaction is direct (PubMed:12509254). Interacts with XRCC6/Ku70; the interaction is direct (PubMed:17124166). Interacts with APTX and APLF (PubMed:15380105, PubMed:17396150, PubMed:17353262, PubMed:18077224). Forms a heterotetramer with IFFO1; the interaction involves LIG4-free XRCC4 and leads to the relocalization of IFFO1 to the sites of DNA damage (PubMed:31548606). Interacts with PNKP; mainly interacts with PNKP when phosphorylated at Thr-233, but is also able to interact at much lower level with PNKP when not unphosphorylated (PubMed:15385968, PubMed:20852255, PubMed:28453785). Interacts with POLL (DNA polymerase lambda) (PubMed:30250067). [Protein XRCC4, C-terminus]: Interacts with XKR4; interacts with the processed form of XKR4, which is cleaved by caspase.