Mouse Anti-YWHAB Recombinant Antibody (clone 3D2E10)

CAT#: VS7-0425-WR1144

This product is a mouse IgG2a antibody that binds to human YWHAB. The antibody 3D2E10 recognizes specific amino acid domain of human YWHAB (AA: 1-246). It can be used for IHC, ICC, and FC assays.

Specifications

  • Immunogen
  • Recombinant human YWHAB (AA: 1-246) fragment expressed in E. Coli.
  • Host Species
  • Mouse
  • Type
  • Mouse IgG2a
  • Specificity
  • Human YWHAB
  • Species Reactivity
  • Human
  • Clone
  • 3D2E10
  • Molecule Class
  • Phosphoserine/Phosphothreonine-Binding Protein
  • Applications
  • IHC, ICC, FC
  • Conjugate
  • Unconjugated

Product Property

  • Clonality
  • Monoclonal
  • Purity
  • >95% as determined by SDS-PAGE and SEC-HPLC analysis
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • 0.05% sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
  • Shipping
  • Ice packs

Applications

  • Application Notes
  • Western Blot: 1:500 - 1:2000
    Immunohistochemistry-Paraffin: 1:200 - 1:1000
    Immunocytochemistry: 1:200 - 1:1000
    Flow Cytometry: 1:200 - 1:400
    Enzyme-linked Immunosorbent Assay: 1:10000

Target

  • Alternative Names
  • Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein Beta; Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein, Alpha Polypeptide; Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein, Beta Polypeptide; 14-3-3 Alpha; Protein 1054; KCIP-1; Protein Kinase C Inhibitor Protein-1; Protein Kinase C Inhibitor Protein 1; Brain Protein 14-3-3, Beta Isoform
  • Long Name
  • Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein Beta
  • Cellular Localization
  • Cytoplasm; Membrane; Vacuole
  • Post Translation Modifications
  • Acetylation; Isopeptide bond; Nitration; Phosphoprotein; Ubl conjugation
  • Function
  • Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Negative regulator of signaling cascades that mediate activation of MAP kinases via AKAP13.
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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

MSDS

COA

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Protocol & Troubleshooting

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Isotype Control

Secondary Antibody

Reconstitution Buffer

Recommended Dilution Buffer

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