Mouse Anti-YWHAB Recombinant Antibody (clone 3D2E10)
CAT#: VS7-0425-WR1144
This product is a mouse IgG2a antibody that binds to human YWHAB. The antibody 3D2E10 recognizes specific amino acid domain of human YWHAB (AA: 1-246). It can be used for IHC, ICC, and FC assays.
Specifications
- Immunogen
- Recombinant human YWHAB (AA: 1-246) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human YWHAB
- Species Reactivity
- Human
- Clone
- 3D2E10
- Molecule Class
- Phosphoserine/Phosphothreonine-Binding Protein
- Applications
- IHC, ICC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Immunocytochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein Beta; Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein, Alpha Polypeptide; Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein, Beta Polypeptide; 14-3-3 Alpha; Protein 1054; KCIP-1; Protein Kinase C Inhibitor Protein-1; Protein Kinase C Inhibitor Protein 1; Brain Protein 14-3-3, Beta Isoform
- Gene ID
- 7529
- UniProt ID
- P31946
- Long Name
- Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein Beta
- Cellular Localization
- Cytoplasm; Membrane; Vacuole
- Post Translation Modifications
- Acetylation; Isopeptide bond; Nitration; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_003395.1
- Function
- Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Negative regulator of signaling cascades that mediate activation of MAP kinases via AKAP13.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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CAT | Product Name | Application | Type |
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CAT | Product Name | Application | Type |
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