Mouse Anti-YWHAZ Recombinant Antibody (clone 19G7E9)
CAT#: ZG-0254U
Mouse anti-YWHAZ antibody (clone 19G7E9) displayed high specificity against the YWHAZ protein of Human, Mouse and Rat.
Tested Data
WB
Figure 1 Mouse Anti-YWHAZ Antibody (ZG-0254U) in WB
Positive WB detected in: NIH/3T3 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, RAW264.7 whole cell lysate, Rat Brain tissue All lanes YWHAZ antibody at 1: 5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 34 KDa
Observed band size: 34 KDa
Exposure time: 1min
IHC
Figure 2 Mouse Anti-YWHAZ Antibody (ZG-0254U) in IHC
IHC image of ZG-0254U diluted at 1:200 and staining in paraffin-embedded human lung cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37°C. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.
IF
Figure 3 Mouse Anti-YWHAZ Antibody (ZG-0254U) in IF
Immunofluorescence staining of U251 cells with ZG-0254U at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Mouse IgG (H+L).
FC
Figure 4 Mouse Anti-YWHAZ Antibody (ZG-0254U) in FC
Overlay histogram showing HepG2 cells stained with ZG-0254U (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (1µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.
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Specifications
- Immunogen
- Recombinant Human 14-3-3 protein zeta/delta protein (133-212AA)
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human YWHAZ
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 19G7E9
- Applications
- ELISA, WB, IHC, IF, FC
- Conjugate
- Unconjugated
- Related Disease
- Ophthalmia Nodosa and Williams-Beuren Syndrome
Product Property
- Purification
- Protein A purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
- Preservative
- 0.03% Proclin 300
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry. Specific conditions should be optimized by the end user.
WB:1:5000-160000, IHC:1:200, IF:1:50, FC:1:100
Target
- Alternative Names
- HEL4; YWHAD; KCIP-1; HEL-S-3; POPCHAS; HEL-S-93; 14-3-3-zeta; 14-3-3 protein zeta/delta; 14-3-3 delta; 14-3-3 protein/cytosolic phospholipase A2; 14-3-3 zeta; epididymis luminal protein 4; epididymis secretory protein Li 3; epididymis secretory protein Li 93; phospholipase A2; protein kinase C inhibitor protein-1; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, delta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein, zeta polypeptide
- Gene ID
- 7534
- UniProt ID
- P63104
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "YWHAZ"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2849z | Mouse Anti-YWHAZ Recombinant Antibody (clone 34H5) | WB, IF, IHC | Mouse IgG2b, κ |
| VS3-QX1260 | Mouse Anti-YWHAZ Recombinant Antibody (clone 1314CT423.108.153.173.140) | WB, ICC | Mouse IgG2b |
| VS3-CJ1100 | Mouse Anti-YWHAZ Recombinant Antibody (VS3-CJ1100) | WB, IF | Mouse IgG2b, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3908 | Rabbit Anti-YWHAZ Recombinant Antibody (clone DS3908AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2514 | Anti-YWHAZ Immunohistochemistry Kit | IHC | |
| VS-0525-XY7996 | Anti-Human YWHAZ Immunohistochemistry Kit | IHC |
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