Rabbit Anti-ZEB1 Recombinant Antibody (clone R01-5A7) (CAT#: VS3-XY3543)
It is a recombinant rabbit antibody specific for ZEB1 (Zinc finger E-box binding homeobox 1), which acts as a transcriptional repressor and inhibits interleukin-2 (IL-2) gene expression. This antibody specifically targets human ZEB1. This product has been tested using Western Blot, Immunohistochemistry-Paraffin, Flow cytometry, Immunocytochemistry or Immunofluorescence. The recommended dilutions of Western Blot, Immunohistochemistry-Paraffin, Flow cytometry and Immunofluorescence is 1/50-1/100, 1/50-1/100, 1/50-1/100 and 1/50-1/200, respectively.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27524/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27524/if_selected.jpg
Figure 2 IHC staining of human smooth muscle
(Immunohistochemical staining of human smooth muscle shows strong nuclear positivity in smooth muscle cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79943/170691_B_6_7_selected.jpg
(Immunohistochemical staining of human smooth muscle shows strong nuclear positivity in smooth muscle cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79943/170691_B_6_7_selected.jpg
Figure 3 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27524/218_C10_1_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27524/218_C10_1_red_green.jpg
Figure 4 IF staining of human cell line A549
(Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79943/2000_B6_1_red_green.jpg
(Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79943/2000_B6_1_red_green.jpg
Figure 5 Cerebral cortex
(Endothelial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58686/125920_B_9_5.jpg
(Endothelial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58686/125920_B_9_5.jpg
Figure 6 Colon
(Endothelial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58686/125920_A_9_3.jpg
(Endothelial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58686/125920_A_9_3.jpg
Figure 7 Kidney
(Cells in glomeruli
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58686/125920_A_8_5.jpg
(Cells in glomeruli
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58686/125920_A_8_5.jpg
Figure 8 Testis
(Peritubular cells
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58686/125920_A_6_6.jpg
(Peritubular cells
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58686/125920_A_6_6.jpg
Figure 9 Smooth muscle
(Smooth muscle cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58686/125920_B_6_7.jpg
(Smooth muscle cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58686/125920_B_6_7.jpg
Figure 10 RNA cell line category: Cell line enhanced (ASC TERT1, TIME)
(Cell lines ordered by descending RNA expression order.)
* https://v21.proteinatlas.org/ENSG00000148516-ZEB1
(Cell lines ordered by descending RNA expression order.)
* https://v21.proteinatlas.org/ENSG00000148516-ZEB1
Figure 10 RNA cell line category: Cell line enhanced (ASC TERT1, TIME)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000148516-ZEB1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000148516-ZEB1
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ZEB1
- Species Reactivity
- Human
- Clone
- R01-5A7
- Applications
- WB, IHC-P, ICC, IF, FC
- Related Disease
- Corneal Dystrophy, Fuchs Endothelial, 6; Corneal Dystrophy, Posterior Polymorphous, 3
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
FC: 1/50-1/100
Target
- Alternative Names
- BZP; TCF8; AREB6; FECD6; NIL2A; PPCD3; ZFHEP; ZFHX1A; DELTAEF1
- Gene ID
- 6935
- UniProt ID
- P37275
- Long Name
- Zinc finger E-box binding homeobox 1
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Ubiquitination at Lys195 (NX_P37275 [NX_P37275-1])
Modification sites at PhosphoSitePlus (P37275)
Glycosylation from GlyGen (P37275) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_047281639.1
- Function
- Acts as a transcriptional repressor.
Inhibits interleukin-2 (IL-2) gene expression.
Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-693 | Human Anti-ZEB1 Recombinant Antibody (clone U20) | IP, IF, ELISA | Human IgG |
| MOB-0682MZ | Recombinant Mouse Anti-Human ZEB1 Antibody (clone 527B8I20) | WB | Mouse antibody |
| MOB-120LC | Mouse Anti-ZEB1 Recombinant Antibody (clone 2A8A6) | WB, FC | Mouse IgG |
| VS3-CJ921 | Rabbit Anti-ZEB1 Recombinant Antibody (clone H-102) | WB, IF, IP | Rabbit IgG |
| VS3-XY1617 | Mouse Anti-ZEB1 Recombinant Antibody (clone 2A8H3) | ELISA, WB, IHC, FC, ICC | Mouse IgG1 |
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