Rabbit Anti-ZEB1 Recombinant Antibody (clone R01-5A7)
CAT#: VS3-XY3543
It is a recombinant rabbit antibody specific for ZEB1 (Zinc finger E-box binding homeobox 1), which acts as a transcriptional repressor and inhibits interleukin-2 (IL-2) gene expression. This antibody specifically targets human ZEB1. This product has been tested using Western Blot, Immunohistochemistry-Paraffin, Flow cytometry, Immunocytochemistry or Immunofluorescence. The recommended dilutions of Western Blot, Immunohistochemistry-Paraffin, Flow cytometry and Immunofluorescence is 1/50-1/100, 1/50-1/100, 1/50-1/100 and 1/50-1/200, respectively.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27524/if_selected.jpg
Subcellular Location
Figure 2 IHC staining of human smooth muscle
Immunohistochemical staining of human smooth muscle shows strong nuclear positivity in smooth muscle cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79943/170691_B_6_7_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27524/218_C10_1_red_green.jpg
Subcellular Location
Figure 4 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79943/2000_B6_1_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58686/125920_B_9_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58686/125920_A_9_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58686/125920_A_8_5.jpg
Normal Tissue
Figure 8 Testis
Peritubular cells
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58686/125920_A_6_6.jpg
Normal Tissue
Figure 9 Smooth muscle
Smooth muscle cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58686/125920_B_6_7.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (ASC TERT1, TIME)
Cell lines ordered by descending RNA expression order.
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (ASC TERT1, TIME)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000148516-ZEB1
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ZEB1
- Species Reactivity
- Human
- Clone
- R01-5A7
- Applications
- WB, IHC-P, ICC, IF, FC
- Related Disease
- Corneal Dystrophy, Fuchs Endothelial, 6; Corneal Dystrophy, Posterior Polymorphous, 3
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
FC: 1/50-1/100
Target
- Alternative Names
- BZP; TCF8; AREB6; FECD6; NIL2A; PPCD3; ZFHEP; ZFHX1A; DELTAEF1
- Gene ID
- 6935
- UniProt ID
- P37275
- Long Name
- Zinc finger E-box binding homeobox 1
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Ubiquitination at Lys195 (NX_P37275 [NX_P37275-1])
Modification sites at PhosphoSitePlus (P37275)
Glycosylation from GlyGen (P37275) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_047281639.1
- Function
- Acts as a transcriptional repressor.
Inhibits interleukin-2 (IL-2) gene expression.
Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "ZEB1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-693 | Human Anti-ZEB1 Recombinant Antibody (clone U20) | IP, IF, ELISA | Human IgG |
| MOB-0682MZ | Recombinant Mouse Anti-Human ZEB1 Antibody (clone 527B8I20) | WB | Mouse antibody |
| MOB-120LC | Mouse Anti-ZEB1 Recombinant Antibody (clone 2A8A6) | WB, FC | Mouse IgG |
| VS3-XY1617 | Mouse Anti-ZEB1 Recombinant Antibody (clone 2A8H3) | ELISA, WB, IHC, FC, ICC | Mouse IgG1 |
| VS13-YC1239 | Rabbit Anti-ZEB1 Recombinant Antibody (VS13-YC1239) | IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY8008 | Anti-ZEB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY8009 | Anti-Mouse ZEB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY8010 | Anti-Canine ZEB1 Immunohistochemistry Kit | IHC |
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