Human Anti-CXCL13 (clone W253B4) scFv-Fc Chimera
CAT#: VS-0125-FY12
The scFv-Fc format integrates single-chain variable fragments (scFvs) with an Fc domain, enhancing the molecule's half-life in circulation and stability. A flexible (A4T)3 linker is employed to ensure optimal spatial orientation and functionality of the scFv domains, thereby preserving their biological activity while bolstering overall stability. Additionally, advancements in affinity for both human and cynomolgus CXCL13 have been achieved through targeted mutagenesis and selection. These improvements are essential for the therapeutic efficacy of the product, positioning it as a promising option in targeted therapies.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human bone marrow
Immunohistochemical staining of human bone marrow shows cytoplasmic positivity in hematopoietic cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52613/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52613/126780_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52613/126780_A_9_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52613/126780_A_7_5.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52613/126780_A_4_6.jpg
Normal Tissue
Figure 6 Bone marrow
Hematopoietic cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52613/126780_B_5_4.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (U-266/70)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000156234-CXCL13
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Specifications
- Host Species
- Human
- Type
- Human IgG1, scFv-Fc
- Species Reactivity
- Human, Cynomolgus
- Clone
- W253B4
- Applications
- ELISA, Neutralization
- Related Disease
- Cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Stabilizer
- No Stabilizers
- Sterility
- 0.2 μM filtered
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- The anti-CXCL13 scFv-Fc is engineered to neutralize the chemokine CXCL13 by binding to it and preventing interaction with the CXCR5 receptor. This action plays a crucial role in modulating immune responses, as CXCL13 is known to recruit immune cells to inflamed or tumor sites. Additionally, its tetravalent bispecific format allows it to engage multiple targets, potentially enhancing therapeutic effectiveness through synergistic immune modulation. The design improvements made to the molecule focus on stability and manufacturability. These enhancements facilitate the formulation of the scFv-Fc in high concentrations. Consequently, it is suitable for subcutaneous delivery, which offers a more patient-friendly administration route compared to intravenous delivery. Overall, the anti-CXCL13 scFv-Fc holds promise in advancing immune therapies.
Target
- Alternative Names
- C-X-C Motif Chemokine Ligand 13; Small Inducible Cytokine B Subfamily (Cys-X-Cys Motif), Member 13 (B-Cell Chemoattractant); B Cell-Attracting Chemokine 1; B Lymphocyte Chemoattractant; Small-Inducible Cytokine B13; B-Cell Chemoattractant; CXC Chemokine BLC; SCYB13; ANGIE; BCA-1; BCA1
- Gene ID
- 10563
- UniProt ID
- O43927
- Sequence Similarities
- Belongs to the intercrine alpha (chemokine CxC) family.
- Cellular Localization
- Secreted
- Post Translation Modifications
- Modification sites at PhosphoSitePlus
Modification sites at neXtProt
- Protein Refseq
- NP_001358487.1; NP_006410.1
- Function
- Chemotactic for B-lymphocytes but not for T-lymphocytes, monocytes and neutrophils.
Does not induce calcium release in B-lymphocytes.
Binds to BLR1/CXCR5.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
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|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-104MZ | Anti-Human CXCL13 Recombinant Antibody (MAb 1476) | ELISA | Chimeric antibody (mouse/human) |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-105MZ | Anti-Human CXCL13 Recombinant Antibody (MAb 5080) | ELISA | Humanized antibody |
| TAB-106MZ | Anti-Human CXCL13 Recombinant Antibody (MAb 5261) | ELISA | Humanized antibody |
| TAB-105MZ-F(E) | Anti-Human CXCL13 Recombinant Antibody Fab Fragment (MAb 5080) | ELISA | Humanized antibody |
| TAB-106MZ-F(E) | Anti-Human CXCL13 Recombinant Antibody Fab Fragment (MAb 5261) | ELISA | Humanized antibody |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-108MZ | Anti-Human CXCL13 Recombinant Antibody | cAMP assay | Human antibody |
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| TAB-108MZ-F(E) | Anti-Human CXCL13 Recombinant Antibody Fab Fragment | cAMP assay | Human antibody |
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| PABX-041-F (E) | Human Anti-CXCL13 Recombinant Antibody; Fab Fragment (PABX-041-F (E)) | Neut, FuncS | Human Fab |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0060-CN-S(P) | Human Anti-CXCL13 Recombinant Antibody (clone Ab 1); scFv Fragment | FC, ELISA, WB, IF | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0060-CN-F(E) | Human Anti-CXCL13 Recombinant Antibody (clone Ab 1); Fab Fragment | FC, ELISA, WB, IF | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0125-FY62 | Human Anti-CXCL13 (clone E10) scFv-Fc Chimera | IA | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| VS-0525-XY1816 | Anti-Mouse CXCL13 Immunohistochemistry Kit | IHC |
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