Mouse Anti-CCNE1 Recombinant Antibody (clone 5F8B4)
CAT#: VS3-XY184
Clone 5F8B4 is a mouse antibody, which reacts with CCNE1 protein. This antibody is suitable for CCNE1 detection by WB, IHC, FC assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18169/if_selected.jpg
Normal Tissue
Figure 2 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: nuclear Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18169/43894_A_4_6.jpg
Normal Tissue
Figure 3 Placenta
Decidual cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/308/2153_A_1_7.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18169/43894_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16682/36096_A_7_3.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18169/43894_A_9_4.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (BEWO)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000105173-CCNE1
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Specifications
- Immunogen
- Recombinant fragment of human CCNE1 (a.a. 307-410) expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- CCNE1
- Clone
- 5F8B4
- Applications
- Western Blot, Immunohistochemistry, Flow Cytometry
Product Property
- Format
- Liquid
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "CCNE1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B078(A) | Recombinant Anti-human CCNE1 Intrabody [(D-Arg)9] | FACS, ICC, FuncS | scFv-(D-Arg)9 |
| IAB-B078(G) | Recombinant Anti-human CCNE1 Intrabody [+36 GFP] | PCA, WB, FuncS | scFv-Tat |
| IAB-B078(T) | Recombinant Anti-human CCNE1 Intrabody [Tat] | FACS, ELISA, IF, FuncS | scFv-Tat |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0517 | Hi-Affi™ Rabbit Anti-CCNE1 Recombinant Antibody (clone DS517AB) | FC, IF, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0415-CN | Rabbit Anti-CCNE1 Recombinant Antibody (clone CBACN-166) | WB, IF | Rabbit IgG |
| MRO-2315-CN | Rabbit Anti-CCNE1 Recombinant Antibody (clone CBACN-607) | WB, IF, IHC | Rabbit IgG |
| ZG-0683F | Mouse Anti-CCNE1 Recombinant Antibody (clone 168) | WB, IP, WB | Mouse IgG2a |
| ZG-0019U | Rabbit Anti-CCNE1 Recombinant Antibody (clone 3A5) | ELISA, IHC, IF | Rabbit IgG |
| ZG-0074U | Rabbit Anti-CCNE1 Recombinant Antibody (clone 9H12) | ELISA, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1090 | Anti-CCNE1 Immunohistochemistry Kit | IHC |
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