Mouse Anti-CDKN2A Recombinant Antibody (clone 2D9A12)
CAT#: VS3-XY485
Clone 2D9A12 is a mouse antibody, which reacts with CDKN2A protein. This antibody is suitable for CDKN2A detection by ELISA, WB, IHC assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to nucleoli.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47838/979_F9_4_selected.jpg
Normal Tissue
Figure 2 IHC staining of human stomach
Immunohistochemical staining of human stomach shows moderate nuclear and cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/93/ihc_selected.jpg
Normal Tissue
Figure 3 Tonsil
High expression in tonsil
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/445/2553_A_4_8_val_selected.jpg
Normal Tissue
Figure 4 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows strong positivity in a subset of cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18232/ihc_selected.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/445/2553_A_8_8.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/445/2553_A_8_5.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (CAPAN-2, HEK93, HeLa, SK-MEL-30)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000147889-CDKN2A
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Specifications
- Immunogen
- Recombinant fragment of CDKN2A expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- CDKN2A
- Species Reactivity
- Human
- Clone
- 2D9A12
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry
Product Property
- Format
- Liquid
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody is recommended for Western Blot (1/500-1/2000), Enzyme-linked Immunosorbent Assay (1/10000) and Immunohistochemistry(1/200-1/1000).
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "Clone 2D9A12"
- CAT
- Product Name
See other products for "CDKN2A"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0116MZ | Chicken Anti-CDKN2A Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0642 | Hi-Affi™ Rabbit Anti-CDKN2A Recombinant Antibody (clone DS642AB) | FC, ICC, IF, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0877LY-S(P) | Mouse Anti-CDKN2A Recombinant Antibody (clone CBL015); scfv Fragment | IHC, WB | Mouse scfv |
| HPAB-M0037-YC-S(P) | Mouse Anti-CDKN2A Recombinant Antibody (clone P1); scFv Fragment | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0877LY-F(E) | Mouse Anti-CDKN2A Recombinant Antibody (clone CBL015); Fab Fragment | IHC, WB | Mouse Fab |
| HPAB-M0037-YC-F(E) | Mouse Anti-CDKN2A Recombinant Antibody (clone P1); Fab Fragment | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0394U | Mouse Anti-CDKN2A Recombinant Antibody (clone R15-A) | IHC-P | Mouse IgG |
| VS3-XY481 | Mouse Anti-CDKN2A Recombinant Antibody (clone 1D7D2) | ELISA, WB, IHC, FC | Mouse IgG1 |
| VS3-XY482 | Mouse Anti-CDKN2A Recombinant Antibody (clone 1D7D2A1) | ELISA, WB, IHC, FC | Mouse IgG1 |
| VS3-XY483 | Mouse Anti-CDKN2A Recombinant Antibody (clone 3B12) | ELISA, WB, IHC, FC | Mouse IgG1 |
| VS3-XY484 | Mouse Anti-CDKN2A Recombinant Antibody (clone 1E12E10) | ELISA, WB, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1355 | AbPlus™ Anti-CDKN2A Magnetic Beads (VS-0724-YC1355) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY414 | Anti-CDKN2A Immunohistochemistry Kit | IHC | |
| VS-0525-XY1341 | Anti-Rat CDKN2A Immunohistochemistry Kit | IHC | |
| VS-0525-XY1340 | Anti-Human CDKN2A Immunohistochemistry Kit | IHC |
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