Mouse Anti-CX3CR1 scFv-Fc Chimera (VS-0625-FY6)
CAT#: VS-0625-FY6
This anti-CX3CR1 scFv-FC is a recombinant fusion protein specifically engineered to target the chemokine receptor CX3CR1. Its single-chain variable fragment (scFv) boasts high affinity for CX3CR1 and directly competes with CX3CL1, effectively blocking its binding by recognizing an epitope at or near the CX3CL1 binding site. This blockade is significant given CX3CR1's crucial role in the migration and function of immune cells like monocytes, macrophages, and NK cells. The inclusion of a human IgG1 Fc domain further enhances its utility.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HEL
Immunofluorescent staining of human cell line HEL shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46587/1813_G11_17_cr59f1a1656f54b_selected.jpg
Normal Tissue
Figure 2 IHC staining of human caudate
Immunohistochemical staining of human caudate shows distinct positivity.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46587/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows strong positivity in a subsets of cells outside reaction centra.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/32478/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46587/139613_B_8_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46587/139613_A_9_3.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46587/139613_A_9_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46587/139613_A_8_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46587/139613_A_6_6.jpg
Normal Tissue
Figure 9 Lymph node
Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46587/139613_A_8_8.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enriched (RPMI-8226)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000168329-CX3CR1
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Specifications
- Immunogen
- The antibodies for mCX3CR1 was generated by immunizing CX3CR1 knockout mice with Jurkat cells transfected with mCX3CR1.
- Host Species
- Mouse
- Type
- Mouse IgG1, scFv-Fc
- Specificity
- The anti-mCX3CR1 monoclonal antibody binds to cell surface-expressed mCX3CR1 (mouse CX3CR1).
- Species Reactivity
- Mouse
- Applications
- Blocking, Flow Cytometry
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Blocking: 2 ug/mL
Flow Cytometry: 10 ug/mL
The optimal dilution concentration is determined by the experiment.
Target
- Alternative Names
- C-X3-C Motif Chemokine Receptor 1; Chemokine (C-X3-C Motif) Receptor 1; Beta Chemokine Receptor-Like 1; Chemokine (C-X3-C) Receptor 1; G-Protein Coupled Receptor 13; Fractalkine Receptor; C-X3-C CKR-1; CMK-BRL-1; CMK-BRL1
- Gene ID
- 1524
- UniProt ID
- P49238
- Long Name
- C-X3-C Motif Chemokine Receptor 1
- Sequence Similarities
- Belongs to the G-protein coupled receptor 1 family.
- Cellular Localization
- Cell membrane
- Post Translation Modifications
- This protein is not N-glycosylated which is unusual for G-protein-coupled receptors.
Modification sites at PhosphoSitePlus
Modification sites at neXtProt
- Protein Refseq
- XP_047303494.1; XP_054201399.1; NP_001164642.1; NP_001164643.1; NP_001164645.1
- Function
- Acts as a regulator of inflammation process leading to atherogenesis by mediating macrophage and monocyte recruitment to inflamed atherosclerotic plaques, promoting cell survival (By similarity).
Involved in airway inflammation by promoting interleukin 2-producing T helper (Th2) cell survival in inflamed lung (By similarity).
Involved in the migration of circulating monocytes to non-inflamed tissues, where they differentiate into macrophages and dendritic cells (By similarity).
Acts as a negative regulator of angiogenesis, probably by promoting macrophage chemotaxis (PubMed:14581400, 18971423).
Plays a key role in brain microglia by regulating inflammatory response in the central nervous system (CNS) and regulating synapse maturation (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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