Mouse Anti-ENO2 Recombinant Antibody (clone 5D3) (CAT#: VS3-XY623)
Clone 5D3 is a mouse antibody, which reacts with human ENO2 protein. This antibody is suitable for ENO2 detection by ELISA, WB, IHC, FC assays.
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We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Normal Tissue
RNA Expression
Figure 1 High expression in cerebral cortex.
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63/ihc_selected.jpg
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63/ihc_selected.jpg
Figure 2 Low expression in kidney.
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63/1316_A_8_5_val_selected.jpg
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63/1316_A_8_5_val_selected.jpg
Figure 3 Low expression in pancreas.
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73539/154876_A_3_3_val_selected.jpg
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73539/154876_A_3_3_val_selected.jpg
Figure 4 Cerebral cortex
(Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63/1726_B_7_5.jpg
(Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63/1726_B_7_5.jpg
Figure 5 Colon
(Enterocytes - Peripheral nerve/ganglion Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63/1316_A_9_3.jpg
(Enterocytes - Peripheral nerve/ganglion Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63/1316_A_9_3.jpg
Figure 6 RNA cell line category: Cell line enhanced (hTERT-RPE1, U-87 MG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000111674-ENO2
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000111674-ENO2
Specifications
- Immunogen
- Recombinant fragment of human ENO2 (a.a. 251-433) expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human ENO2
- Species Reactivity
- Human
- Clone
- 5D3
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry, Immunohistochemistry
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody is recommended for Western Blot (1/500-1/2000), Enzyme-linked Immunosorbent Assay (1/10000), Flow Cytometry (1/200-1/400) and Immunohistochemistry(1/200-1/1000).
Target
- Alternative Names
- Enolase 2; 2-Phospho-D-Glycerate Hydro-Lyase; Enolase 2 (Gamma, Neuronal); Neuronal Enriched Enolase; Neuron-Specific Enolase; Neural Enolase; Gamma-Enolase; EC 4.2.1.11
- Gene ID
- 2026
- UniProt ID
- P09104
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1464CT | Recombinant Mouse anti-Human ENO2 Monoclonal antibody (2D2) | I-ELISA, IHC-P, WB | |
| MRO-1114-CN | Rabbit Anti-ENO2 Recombinant Antibody (clone CBACN-409) | WB, IF, IHC | Rabbit IgG |
| MOB-0145F | Mouse Anti-ENO2 Recombinant Antibody (clone 13E2) | WB, IF, IHC-P | Mouse IgG |
| VS3-CJ534 | Mouse Anti-ENO2 Recombinant Antibody (VS3-CJ534) | WB, IHC-P | Mouse IgG2b, κ |
| VS3-CJ535 | Rabbit Anti-ENO2 Recombinant Antibody (VS3-CJ535) | WB, ICC, IHC, FC | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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