Mouse Anti-ESR1 Recombinant Antibody (clone 2G112B3) (CAT#: VS3-XY653)
Clone 2G112B3 is a mouse antibody, which reacts with human ESR1 protein. This antibody is suitable for ESR1 detection by ELISA, WB assays.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/449/1134_D1_1_selected.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/449/1134_D1_1_selected.jpg
Figure 2 IF staining of human cell line RT4
(Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/449/1423_D4_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/449/1423_D4_1_blue_red_green.jpg
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/449/1_G2_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/449/1_G2_1_blue_red_green.jpg
Figure 4 Endometrium
(Cells in endometrial stroma
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37/288_B_3_3.jpg
(Cells in endometrial stroma
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37/288_B_3_3.jpg
Figure 5 RNA cell line category: Group enriched (MCF7, T-47d)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000091831-ESR1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000091831-ESR1
Specifications
- Immunogen
- Synthetic peptide of human ESR1 corresponding to SLQKYYITGEAEGFPATV
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human ESR1
- Species Reactivity
- Human
- Clone
- 2G112B3
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot
Product Property
- Format
- Liquid
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody is recommended for Western Blot (1/500-1/2000) and Enzyme-linked Immunosorbent Assay (1/10000).
Target
- Alternative Names
- Estrogen Receptor 1; Nuclear Receptor Subfamily 3 Group A Member 1; Oestrogen Receptor Alpha; Estradiol Receptor; E2 Receptor Alpha; ER-Alpha; NR3A1; ESR; ER
- Gene ID
- 2099
- UniProt ID
- P03372
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-898-VHH | Recombinant Anti-Human ESR1 VHH Single Domain Antibody | FA, FC, ICC, ELISA | Llama VHH |
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