Mouse Anti-LYN Recombinant Antibody (clone 2H8D7) (CAT#: VS3-XY1023)
Clone 2H8D7 is a mouse antibody, which reacts with LYN protein. This antibody is suitable for LYN detection by ELISA, WB assays.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1231/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1231/if_selected.jpg
Figure 2 IHC staining of human spleen
(High expression. RNA expression: 85.4 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1231/4032_B_7_4_val_selected.jpg
(High expression. RNA expression: 85.4 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1231/4032_B_7_4_val_selected.jpg
Figure 3 IHC staining of human cerebral cortex
(Low expression. RNA expression: 8.7 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1231/4032_B_8_5_val_selected.jpg
(Low expression. RNA expression: 8.7 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1231/4032_B_8_5_val_selected.jpg
Figure 4 Colon
(Glandular cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1231/4032_A_9_3.jpg
(Glandular cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1231/4032_A_9_3.jpg
Figure 5 Liver
(Cholangiocytes
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4492/12394_A_7_4.jpg
(Cholangiocytes
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4492/12394_A_7_4.jpg
Figure 6 Kidney
(Cells in tubules
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1231/4032_A_9_5.jpg
(Cells in tubules
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1231/4032_A_9_5.jpg
Figure 7 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1231/4032_A_8_8.jpg
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1231/4032_A_8_8.jpg
Figure 8 RNA cell line category: Cell line enhanced (HEL, HMC-1)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000254087-LYN
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000254087-LYN
Specifications
- Immunogen
- Recombinant fragment of LYN expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- LYN
- Species Reactivity
- Human
- Clone
- 2H8D7
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot
Product Property
- Format
- Liquid
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody is recommended for Western Blot (1/500-1/2000) and Enzyme-linked Immunosorbent Assay (1/10000).
Target
Related Resources
BCR Signaling Pathway
FcεR1 Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2781z | Mouse Anti-LYN Recombinant Antibody (clone 33H4) | WB, ICC, IF, IP | Mouse IgG1 |
| MOB-1024MZ | Recombinant Mouse Anti-Human LYN Antibody (clone 3I9E8) | ELISA | Mouse antibody |
| ZG-030R | Mouse Anti-Lyn Recombinant Antibody (ZG-030R) | WB, ELISA | Mouse IgG |
| VS3-CJ478 | Mouse Anti-LYN Recombinant Antibody (VS3-CJ478) | WB | Mouse IgG1, κ |
| VS3-WK2169 | Rabbit Anti-LYN (pY396) Recombinant Antibody (clone R07-3H2) | WB, ICC, IF, FC | Rabbit IgG |
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