Rabbit Anti-HKDC1 Polyclonal Antibody (MRO-1934-CN)
CAT#: MRO-1934-CN
This antibody is a rabbit polyclonal antibody that is capable of binding to HKDC1.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line CACO-2
Immunofluorescent staining of human cell line CACO-2 shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64198/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64230/if_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64198/168497_B_9_5.jpg
Normal Tissue
Figure 4 Small intestine
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64198/168497_A_5_2.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64198/168497_A_9_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64198/168497_A_6_6.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (A549, CACO-2, CAPAN-2, EFO-21, GAMG, Hep G2, RPTEC TERT1)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000156510-HKDC1
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Specifications
- Immunogen
- Recombinant protein within human HKDC1 260-460aa.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human HKDC1 (Hexokinase Domain Containing 1)
- Species Reactivity
- Human, Mouse, Rat
- Applications
- Used for immunoassay techniques such as: Western Blot; Immunofluorescence; Immunohistochemistry; Flow Cytometry
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein affinity purified
- Format
- Liquid
- Buffer
- 1*TBS, pH 7.4, 0.5% BSA, 50% Glycerol
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Target
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Versatile Polyclonal Antibody
This Rabbit Anti-HKDC1 Polyclonal Antibody has been a workhorse in our lab. We have used it for Western Blot, Immunofluorescence, and IHC. It recognizes the target in Human, Mouse, and Rat samples, which is incredibly convenient for our cross-species studies. The affinity purification really helps in reducing background.
Specific HKDC1 Staining
We focused on the Hexokinase Domain Containing 1 protein, and this antibody (immunogen aa 260-460) provided very specific staining in our IF experiments. The antibody arrived in a glycerol buffer that made storage at -20°C convenient. It works reliably across multiple applications including Flow Cytometry.
Q&As
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What species reactivity does this HKDC1 antibody exhibit?
A: This Rabbit Polyclonal Antibody is capable of reacting with HKDC1 from Human, Mouse, and Rat. This cross-reactivity makes it a versatile tool for comparative research across different model organisms. It is validated for WB, IF, IHC, and FC.
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What immunogen was used to generate this antibody?
A: This antibody was generated using a recombinant protein corresponding to a specific region within human HKDC1, spanning amino acids 260-460. It is affinity purified to ensure specificity and is supplied in a buffer containing 50% glycerol for stable storage at -20°C.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "HKDC1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-FY675 | Recombinant Rabbit Anti-HKDC1 Antibody (clone R06-8I4) | WB, IP | Rabbit IgG |
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