Rabbit Anti-Hsp27 (phospho S82) Monoclonal Antibody
CAT#: VS-0126-XL183
Recognizing the critical S32 phosphorylation site of IKB alpha, this rabbit monoclonal provides targeted detection of NF-kappaB activation. It is a robust reagent optimized for biochemical quantification in WB and IP.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47330/if_selected.jpg
Normal Tissue
Figure 2 Esophagus
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4439/17421_B_4_1_val_selected.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Enterocytes Staining: Low Intensity: Moderate Quantity: <25% Enterocytes -
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47331/104843_A_9_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Distal tubules Staining: Medium Intensity: Moderate Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47331/104843_A_7_5.jpg
Normal Tissue
Figure 5 High expression in vagina
Squamous epithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47331/104843_B_2_2.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (hTCEpi, MCF7)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000106211-HSPB1
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Specifications
- Host Species
- Rabbit
- Antibody Isotype
- Rabbit IgG
- Specificity
- Hsp27 (phospho S82)
- Species Reactivity
- Human; Mouse; Rabbit
- Applications
- WB; IP
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Concentrated by applying a robust chromatography approach focused on selective isolation of the target molecules from the raw materials.
- Concentration
- Lot specific
- Storage
- Store at -20˚C
- Shipping
- Ice Packs
Applications
- Application Notes
- WB: 1:500-1:1000
IP: 1:50
Target
- Alternative Names
- Heat shock protein beta-1; HSP28; SRP27; 27 kDa heat shock protein
- UniProt ID
- P04792
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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