Recombinant Mouse Anti-ACKR3 Antibody (clone 5E6C8)
CAT#: VS3-FY432
This product is a recombinant mouse antibody that recognizes ACKR3 protein. This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Immunocytochemistry, Flow Cytometry.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32003/1048_F7_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human placenta
Immunohistochemical staining of human placenta shows cytoplasmic positivity in trophoblasts.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49718/116961_A_2_7_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line HeLa
Immunofluorescent staining of human cell line HeLa shows localization to plasma membrane & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32003/1047_F7_1_blue_red_green.jpg
Normal Tissue
Figure 4 Pancreas
Exocrine glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49718/116961_A_1_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49718/116961_A_9_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49718/116961_A_4_6.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (HeLa, MCF7, RT4, SiHa, U-2197)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000144476-ACKR3
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- ACKR3
- Clone
- 5E6C8
- Applications
- Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Immunocytochemistry, Flow Cytometry
- Conjugate
- Unconjugated
Product Property
- Purity
- >95%
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Immunocytochemistry, Flow Cytometry.
Target
- Alternative Names
- Atypical Chemokine Receptor 3; Chemokine Orphan Receptor 1; G-Protein Coupled Receptor RDC1 Homolog; Chemokine (C-X-C Motif) Receptor 7; C-X-C Chemokine Receptor Type 7; G-Protein Coupled Receptor 159; CMKOR1; CXC-R7
- Gene ID
- 57007
- UniProt ID
- P25106
- Sequence Similarities
- Belongs to the G-protein coupled receptor 1 family. Atypical chemokine receptor subfamily.
- Cellular Localization
- Cell membrane, Endosome, Membrane
- Post Translation Modifications
- The Ser/Thr residues in the C-terminal cytoplasmic tail may be phosphorylated.
Ubiquitinated at the Lys residues in its C-terminal cytoplasmic tail and is essential for correct trafficking from and to the cell membrane. Deubiquitinated by CXCL12-stimulation in a reversible manner.
- Protein Refseq
- NP_064707.1
- Function
- Atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis. Also known as interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. Acts as a receptor for chemokines CXCL11 and CXCL12/SDF1 (PubMed:16107333, PubMed:19255243, PubMed:19380869, PubMed:20161793, PubMed:22300987).
Chemokine binding leads to ligand internalization and activation of MAPK signaling pathway (PubMed:16940167, PubMed:18653785, PubMed:20018651).
Required for regulation of CXCR4 protein levels in migrating interneurons, thereby adapting their chemokine responsiveness (PubMed:16940167, PubMed:18653785).
In glioma cells, transduces signals via MEK/ERK pathway, mediating resistance to apoptosis. Promotes cell growth and survival (PubMed:16940167, PubMed:20388803).
Not involved in cell migration, adhesion or proliferation of normal hematopoietic progenitors but activated by CXCL11 in malignant hemapoietic cells, leading to phosphorylation of ERK1/2 (MAPK3/MAPK1) and enhanced cell adhesion and migration (PubMed:17804806, PubMed:18653785, PubMed:19641136, PubMed:20887389).
Plays a regulatory role in CXCR4-mediated activation of cell surface integrins by CXCL12 (PubMed:18653785).
Required for heart valve development (PubMed:17804806).
Regulates axon guidance in the oculomotor system through the regulation of CXCL12 levels (PubMed:31211835).
(Microbial infection) Acts as coreceptor with CXCR4 for a restricted number of HIV isolates.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
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| MOB-34 | Recombinant Anti-human ACKR3 Antibody | ELISA, WB, FC, FuncS | IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-34-F(E) | Recombinant Anti-human ACKR3 Antibody Fab Fragment | IF, FuncS | Fab |
| MHH-34-F(E) | Recombinant Human Anti-human ACKR3 Antibody Fab Fragment | ELISA, IF, FuncS | Fab |
| CAT | Product Name | Application | Type |
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| MOB-34-S(P) | Recombinant Anti-human ACKR3 Antibody scFv Fragment | ELISA, WB, FC, FuncS | scFv |
| MHH-34-S(P) | Recombinant Human Anti-human ACKR3 Antibody scFv Fragment | ELISA, WB, IP, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| VS-0325-FY134 | Mouse Anti-ACKR3 scFv-Fc Chimera (VS-0325-FY134) | FC | Mouse IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
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| VS-0425-YC227 | Recombinant Anti-ACKR3 Vesicular Antibody, EV Displayed (VS-0425-YC227) | ELISA, FC, Cell-uptake |
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