Recombinant Rabbit Anti-ACTA1 Antibody (clone R03-5I2)
CAT#: VS3-FY23
This product is a recombinant rabbit antibody that recognizes human and rat ACTA1 protein. It can detect a band of approximately 42 kDa (observedd molecular weight: 42 kDa).
Gene Expression
Tested Data
Normal Tissue
Figure 1 High expression in skeletal muscle.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45/300_B_2_6_val_selected.jpg
Normal Tissue
Figure 2 Low expression in skin 1.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45/300_B_9_1_val_selected.jpg
Normal Tissue
Figure 3 Heart muscle
Cardiomyocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45/300_B_5_6.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enriched (BEWO)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000143632-ACTA1
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WB
Figure 1. Recombinant Rabbit Anti-ACTA1 Antibody (clone R03-5I2) in WB.
Western blot analysis of muscle actin from HeLa lysates using a muscle actin antibody.
IHC
Figure 2. Recombinant Rabbit Anti-ACTA1 Antibody (clone R03-5I2) in IHC.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using an antibody to muscle actin. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Specifications
- Immunogen
- A synthetic peptide of human muscle Actin
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat ACTA1
- Species Reactivity
- Human, Rat
- Clone
- R03-5I2
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 42 kDa; Observed MW: 42 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Actin, Alpha 1, Skeletal Muscle; Nemaline Myopathy Type 3; ACTA; Actin, Alpha Skeletal Muscle; Alpha-Actin-1; CFTD1; CFTDM; ASMA
- Gene ID
- 58
- UniProt ID
- P68133
- Sequence Similarities
- Belongs to the actin family.
- Cellular Localization
- Cytoplasm, Cytoskeleton
- Post Translation Modifications
- Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promotes actin repolymerization.
Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.
Methylated at His-75 by SETD3.
(Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-52 of one monomer and Glu-272 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515).
The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148).
- Protein Refseq
- NP_001091.1
- Function
- Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "ACTA1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-006 | Recombinant Human Anti-ACTA1 Antibody (C2507) | WB, ELISA, IF, FuncS | IgG |
| PABX-006-F (E) | Recombinant Human Anti-ACTA1 Antibody Fab Fragment (C2507) | WB, ELISA, IF, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-006-S (P) | Recombinant Human Anti-ACTA1 Antibody scFv Fragment (C2507) | WB, ELISA, IF, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0047 | Hi-Affi™ Rabbit Anti-ACTA1 Recombinant Antibody (clone DS47AB) | WB, IHC-P, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC3038 | PE-Gogo-B*0101/Human ACTA1 (KRGILTLKY) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0181C | Mouse Anti-ACTA1 Recombinant Antibody (clone 4B11) | WB, IHC-P, IF, IP | Mouse IgG |
| ZG-0527J | Rabbit Anti-ACTA1 Recombinant Antibody (clone 25E3) | ELISA, WB, IHC, IF, FC | Rabbit IgG |
| VS3-QX8 | Mouse Anti-ACTA1 Recombinant Antibody (clone 3B8-5F1-5B2) | IHC | Mouse IgG1 |
| VS3-WK1267 | Mouse Anti-ACTA1 Recombinant Antibody (clone 1E12) | WB | Mouse IgG1 |
| VS3-FY2619 | Rabbit Anti-ACTA1 Recombinant Antibody (VS3-FY2619) | WB, IHC, ICC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY3 | AbPlus™ Anti-ACTA1 Magnetic Beads (JF47-01) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY105 | Anti-ACTA1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY106 | Anti-Rat ACTA1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY107 | Anti-Mouse ACTA1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY108 | Anti-Canine ACTA1 Immunohistochemistry Kit | IHC |
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