Recombinant Mouse Anti-BCL2 Antibody (clone 3B5H7)
CAT#: VS3-FY148
This product is a recombinant mouse antibody that recognizes BCL2 protein. This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line SK-MEL-30
Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm & nuclear membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55295/1484_G3_6_selected.jpg
Normal Tissue
Figure 2 Thyroid gland
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3/155167_B_3_5.jpg
Normal Tissue
Figure 3 Colon
Mucosal lymphoid cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3/155167_A_7_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3/155167_A_8_5.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3/155167_A_7_8.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (HDLM-2, HL-60, Karpas-707, U-266/70)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000171791-BCL2
❮
❯
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- BCL2
- Clone
- 3B5H7
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry
- Conjugate
- Unconjugated
Product Property
- Purity
- >95%
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry.
Target
- Alternative Names
- BCL2, Apoptosis Regulator; Protein Phosphatase 1, Regulatory Subunit 50; B-Cell CLL/Lymphoma 2; Apoptosis Regulator Bcl-2; PPP1R50; Bcl-2
- Gene ID
- 596
- UniProt ID
- P10415
- Cellular Localization
- Cytoplasm, Endoplasmic reticulum, Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus
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Related Signaling Pathways
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
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