Recombinant Rabbit Anti-BUB1B Antibody (clone R05-3G7)
CAT#: VS3-FY172
This product is a recombinant rabbit antibody that recognizes human and hamster BUB1B protein. It can detect a band of approximately 120 kDa (observedd molecular weight: 120 kDa).
Tested Data
Gene Expression
WB
Figure 1. Recombinant Rabbit Anti-BUB1B Antibody (clone R05-3G7) in WB.
Western blot analysis of BubR1 in CHO-K1, Ramos lysates using BUBR1 Antibody.
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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8419/68_H6_1_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000156970-BUB1B
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Specifications
- Immunogen
- A synthetic peptide of human BubR1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Hamster BUB1B
- Species Reactivity
- Human, Hamster
- Clone
- R05-3G7
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 120 kDa; Observed MW: 120 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- BUB1 Mitotic Checkpoint Serine/Threonine Kinase B; MAD3/BUB1-Related Protein Kinase; Mitotic Checkpoint Kinase MAD3L; HBUBR1; BUBR1; MAD3L; SSK1; Budding Uninhibited By Benzimidazoles 1 (Yeast Homolog), Beta; Budding Uninhibited By Benzimidazoles 1 Homolog Beta (Yeast)
- Gene ID
- 701
- UniProt ID
- O60566
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "BUB1B"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2965z | Mouse Anti-BUB1B Recombinant Antibody (clone 11G9) | WB, ICC, IF, IP | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0945MZ | Mouse Anti-BUB1B Recombinant Antibody (clone CBLS-180) | IHC-P | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0396 | Hi-Affi™ Rabbit Anti-BUB1B Recombinant Antibody (clone DS396AB) | WB, IHC-P, FC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1702-CN | Rabbit Anti-BUB1B Polyclonal Antibody (MRO-1702-CN) | WB, IF, IHC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0946F | Sheep Anti-Bub1b Antibody (ZG-0946F) | WB | Sheep IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0575J | Rabbit Anti-BUB1B Recombinant Antibody (clone 7H4) | ELISA, WB, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY269 | Anti-BUB1B Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY862 | Anti-Mouse BUB1B Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY861 | Anti-Human BUB1B Immunohistochemistry Kit | IHC |
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