Recombinant Rabbit Anti-CCNB1 Antibody (clone R05-3B3) (CAT#: VS3-FY215)
This product is a recombinant rabbit antibody that recognizes human CCNB1 protein. It can detect a band of approximately 48 kDa (observedd molecular weight: 55 kDa).
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WB
Figure 1. Recombinant Rabbit Anti-CCNB1 Antibody (clone R05-3B3) in WB.
Western blot analysis of Cyclin B1 (Phospho-Ser126) in HeLa, Ramos lysates using Phosphocyclin B1 (Ser126) Antibody.
Specifications
- Immunogen
- A synthetic phosphopeptide corresponding to residues surrounding Ser126 of human Cyclin B1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CCNB1
- Species Reactivity
- Human
- Clone
- R05-3B3
- Applications
- Western Blot, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 48 kDa; Observed MW: 55 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- Cyclin B1; CCNB; G2/Mitotic-Specific Cyclin B1; G2/Mitotic-Specific Cyclin-B1
- Gene ID
- 891
- UniProt ID
- P14635
- Sequence Similarities
- Belongs to the cyclin family. Cyclin AB subfamily.
- Cellular Localization
- Cytoplasm, Cytoskeleton, Nucleus
- Post Translation Modifications
- Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-147 was also reported to be mediated by PLK1 but Ser-133 seems to be the primary phosphorylation site.
- Protein Refseq
- NP_114172.1
- Function
- Essential for the control of the cell cycle at the G2/M (mitosis) transition.
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2074MZ | Mouse Anti-CCNB1 Recombinant Antibody (clone jmw-qipt5.2) | IF, IP, WB | Mouse IgG |
| VS3-CJ654 | Rabbit Anti-CCNB1 Recombinant Antibody (VS3-CJ654) | WB, ICC, IF, IHC, IP | Rabbit IgG |
| VS3-XY180 | Mouse Anti-CCNB1 Recombinant Antibody (clone 1B10) | ELISA, WB, IHC | Mouse IgG1 |
| VS3-XY181 | Mouse Anti-CCNB1 Recombinant Antibody (clone 5G6) | ELISA, WB, ICC, FC | Mouse IgG1 |
| VS4-WK70 | Mouse Anti-CCNB1 Recombinant Antibody (VS4-WK70) | ELISA, IHC | Mouse IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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