Recombinant Rabbit Anti-CCNB1 Antibody (clone R05-3B3)
CAT#: VS3-FY215
This product is a recombinant rabbit antibody that recognizes human CCNB1 protein. It can detect a band of approximately 48 kDa (observedd molecular weight: 55 kDa).
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30741/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61448/1671_B6_5_selected.jpg
Normal Tissue
Figure 3 Colon
Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: Medium Intensity: Strong Quantity: <25% Enterocytes -
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3804/10580_A_9_3.jpg
Normal Tissue
Figure 4 Testis
Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Strong Quantity: <25% Spermatogonia cells Staining: Medium Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3804/10580_A_4_6.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3804/10580_A_8_8.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000134057-CCNB1
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WB
Figure 1. Recombinant Rabbit Anti-CCNB1 Antibody (clone R05-3B3) in WB.
Western blot analysis of Cyclin B1 (Phospho-Ser126) in HeLa, Ramos lysates using Phosphocyclin B1 (Ser126) Antibody.
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Specifications
- Immunogen
- A synthetic phosphopeptide corresponding to residues surrounding Ser126 of human Cyclin B1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CCNB1
- Species Reactivity
- Human
- Clone
- R05-3B3
- Applications
- Western Blot, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 48 kDa; Observed MW: 55 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- Cyclin B1; CCNB; G2/Mitotic-Specific Cyclin B1; G2/Mitotic-Specific Cyclin-B1
- Gene ID
- 891
- UniProt ID
- P14635
- Sequence Similarities
- Belongs to the cyclin family. Cyclin AB subfamily.
- Cellular Localization
- Cytoplasm, Cytoskeleton, Nucleus
- Post Translation Modifications
- Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-147 was also reported to be mediated by PLK1 but Ser-133 seems to be the primary phosphorylation site.
- Protein Refseq
- NP_114172.1
- Function
- Essential for the control of the cell cycle at the G2/M (mitosis) transition.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "CCNB1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1647z | Mouse Anti-CCNB1 Recombinant Antibody (clone 28C1) | WB, IF, IHC, IP, FuncS | Mouse IgG1, κ |
| ZG-0445F | Mouse Anti-CCNB1 Recombinant Antibody (ZG-0445F) | WB, IHC, ELISA | Mouse IgG |
| VS3-FY213 | Recombinant Rabbit Anti-Ccnb1 Antibody (clone R08-9B7) | WB, ICC, IF | Rabbit IgG |
| VS3-FY214 | Recombinant Rabbit Anti-CCNB1 Antibody (clone R01-7H7) | WB, ICC, IF, IP | Rabbit IgG |
| VS3-WK2269 | Rabbit Anti-CCNB1 (pS126) Recombinant Antibody (VS3-WK2269) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0514 | Hi-Affi™ Rabbit Anti-CCNB1 Recombinant Antibody (clone DS514AB) | FC, ICC, IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC4322 | PE-B*57:01/Human CCNB1 (FAFVTDNTY) MHC Tetramer | FCM | |
| MHC-LC4323 | PE-A*29:02/Human CCNB1 (FAFVTDNTY) MHC Tetramer | FCM | |
| MHC-LC4324 | PE-B*58:01/Human CCNB1 (FAFVTDNTY) MHC Tetramer | FCM | |
| MHC-LC4410 | PE-A*01:01/Human CCNB1 (CSEYVKDIY) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY146 | Mouse Anti-NHP CCNB1 Recombinant Antibody (clone V152) | WB, IHC, IF, IP | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY334 | Anti-CCNB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1081 | Anti-Mouse CCNB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1080 | Anti-Human CCNB1 Immunohistochemistry Kit | IHC |
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