Recombinant Rabbit Anti-CCNE1 Antibody (clone R08-2A1)
CAT#: VS3-FY222
This product is a recombinant rabbit antibody that recognizes human CCNE1 protein. It can detect a band of approximately 47 kDa (observedd molecular weight: 47 kDa).
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18169/if_selected.jpg
Normal Tissue
Figure 2 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: nuclear Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18169/43894_A_4_6.jpg
Normal Tissue
Figure 3 Placenta
Decidual cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/308/2153_A_1_7.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18169/43894_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16682/36096_A_7_3.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18169/43894_A_9_4.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (BEWO)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000105173-CCNE1
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WB
Figure 1. Recombinant Rabbit Anti-CCNE1 Antibody (clone R08-2A1) in WB.
Western blot analysis of Cyclin E1 in K562, Hela lysates using Cyclin E1 Antibody.
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Specifications
- Immunogen
- A synthetic peptide of human Cyclin E1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CCNE1
- Species Reactivity
- Human
- Clone
- R08-2A1
- Applications
- Western Blot
- Conjugate
- Unconjugated
- MW
- Calculated MW: 47 kDa; Observed MW: 47 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Cyclin E1; CCNE; G1/S-Specific Cyclin-E1; Cyclin Es; Cyclin Et; PCCNE1
- Gene ID
- 898
- UniProt ID
- P24864
- Sequence Similarities
- Belongs to the cyclin family. Cyclin E subfamily.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Phosphorylation of both Thr-395 by GSK3 and Ser-399 by CDK2 creates a high affinity degron recognized by FBXW7, and accelerates degradation via the ubiquitin proteasome pathway. Phosphorylation at Thr-77 creates a low affinity degron also recognized by FBXW7.
Ubiquitinated by UHRF2; appears to occur independently of phosphorylation.
- Protein Refseq
- NP_001229.1; NP_001309191.1
- Function
- Essential for the control of the cell cycle at the G1/S (start) transition.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "CCNE1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B078(A) | Recombinant Anti-human CCNE1 Intrabody [(D-Arg)9] | FACS, ICC, FuncS | scFv-(D-Arg)9 |
| IAB-B078(G) | Recombinant Anti-human CCNE1 Intrabody [+36 GFP] | PCA, WB, FuncS | scFv-Tat |
| IAB-B078(T) | Recombinant Anti-human CCNE1 Intrabody [Tat] | FACS, ELISA, IF, FuncS | scFv-Tat |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0517 | Hi-Affi™ Rabbit Anti-CCNE1 Recombinant Antibody (clone DS517AB) | FC, IF, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0415-CN | Rabbit Anti-CCNE1 Recombinant Antibody (clone CBACN-166) | WB, IF | Rabbit IgG |
| ZG-0074U | Rabbit Anti-CCNE1 Recombinant Antibody (clone 9H12) | ELISA, IF | Rabbit IgG |
| VS3-XY184 | Mouse Anti-CCNE1 Recombinant Antibody (clone 5F8B4) | WB, IHC, FC | Mouse IgG1 |
| VS3-XY185 | Mouse Anti-CCNE1 Recombinant Antibody (clone 5F8C5) | ELISA, WB, FC | Mouse IgG1 |
| VS3-WK2145 | Rabbit Anti-CCNE1 (pT77) Recombinant Antibody (clone R02-6U7) | WB, IHC-P, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1090 | Anti-CCNE1 Immunohistochemistry Kit | IHC |
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