Recombinant Rabbit Anti-CHAT Antibody (clone R07-1G8) (CAT#: VS3-FY375)
This product is a recombinant rabbit antibody that recognizes mouse and rat CHAT protein. It can detect a band of approximately 83 kDa (observedd molecular weight: 72 kDa).
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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ICC
Figure 1. Recombinant Rabbit Anti-CHAT Antibody (clone R07-1G8) in ICC.
Immunocytochemical analysis of choline acetyltransferase (green) in SH-SY5Y using choline acetyltransferase antibody and DAPI (blue).
WB
Figure 2. Recombinant Rabbit Anti-CHAT Antibody (clone R07-1G8) in WB.
Western blot analysis of choline acetyltransferase in rat brain lysates using a choline acetyltransferase antibody.
Specifications
- Immunogen
- A synthetic peptide of human Choline Acetyltransferase
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Mouse and Rat CHAT
- Species Reactivity
- Mouse, Rat
- Clone
- R07-1G8
- Applications
- Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 83 kDa; Observed MW: 72 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Choline O-Acetyltransferase; Choline Acetylase; EC 2.3.1.6; CHOACTASE; Acetyl CoA:Choline O-Acetyltransferase; Choline Acetyltransferase
- Gene ID
- 1103
- UniProt ID
- P28329
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1218z | Mouse Anti-CHAT Recombinant Antibody (clone 33G6) | ICC, IF, IHC | Mouse IgG2b |
| MOB-2127MZ | Recombinant Mouse Anti-Human CHAT Antibody | WB | Mouse antibody |
| MRO-0361-CN | Rabbit Anti-CHAT Recombinant Antibody (clone JA67-11) | WB, IP, IF, IHC, FC | Rabbit IgG |
| VS-0223-WK21 | Rabbit Anti-CHAT Recombinant Antibody (VS-0223-WK21) | IHC, IHC-P | Rabbit IgG |
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