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Recombinant Rabbit Anti-CUL1 Antibody (clone R05-3D2)

Recombinant Rabbit Anti-CUL1 Antibody (clone R05-3D2) (CAT#: VS3-FY428)

Name: Recombinant Rabbit Anti-CUL1 Antibody (clone R05-3D2)
SKU: VS3-FY428
Rating: 5 (1 reviews)

This product is a recombinant rabbit antibody that recognizes human, mouse and rat CUL1 protein. It can detect a band of approximately 90 kDa (observedd molecular weight: 90 kDa).

Tested Data
Gene Expression
Datasheet
MSDS
COA

Figure 1. Recombinant Rabbit Anti-CUL1 Antibody (clone R05-3D2) in WB.

Figure 1. Recombinant Rabbit Anti-CUL1 Antibody (clone R05-3D2) in WB.

Western blot analysis of Cullin 1/CUL1 in K562, C6 lysates using Cullin 1/CUL1 Antibody.

ICC

Figure 2. Recombinant Rabbit Anti-CUL1 Antibody (clone R05-3D2) in ICC.

Figure 2. Recombinant Rabbit Anti-CUL1 Antibody (clone R05-3D2) in ICC.

Immunocytochemical analysis of Cullin 1 (green) in HeLa using Cullin 1 Antibody and DAPI (blue).

Subcellular Location

Figure 1 IF staining of human cell line PC-3
(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm & nucleoli.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64584/if_selected.jpg

Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2676/if_selected.jpg

Normal Tissue

Figure 3 IHC staining of human stomach
(Immunohistochemical staining of human stomach shows strong cytoplasmic and weak nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2676/ihc_selected.jpg

Figure 4 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Neuropil Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2676/6646_B_8_5.jpg

Figure 5 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2676/6646_A_7_3.jpg

Figure 6 Testis
(Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: Low Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2676/6646_A_4_6.jpg

RNA Expression

Figure 7 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000055130-CUL1

Datasheet

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
A synthetic peptide of human Cullin 1

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human, Mouse and Rat CUL1

Species Reactivity
Human, Mouse, Rat

Clone
R05-3D2

Applications
Western Blot, Immunocytochemistry, Immunofluorescence

Conjugate
Unconjugated

MW
Calculated MW: 90 kDa; Observed MW: 90 kDa

Product Property

Purification
Affinity Purified

Purity
>95%

Format
Liquid

Buffer
50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA

Preservative
0.01% Sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200

Target

Alternative Names
Cullin 1; CUL-1; Cullin-1

Gene ID
8454

UniProt ID
Q13616

Secondary Antibody

Isotype Control

Related Resources

Related Signaling Pathways

TGF-β Signaling Pathway

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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