Recombinant Mouse Anti-CXCL8 Capture Antibody (clone 4F8)
CAT#: VS3-WK1670
This antibody is a mouse recombinant antibody against human CXCL8. The sandwich ELISA was established using matched antibody pair 4F8 and 10B11. The clone 4F8 can be used in ELISA analysis as a capture antibody at a dilution of 1/1000. It was developed by immunization of mouse with recombinant human CXCL8 protein and then was produced as a recombinant antibody. The isotype of this product is mouse IgG1.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57179/1010_F11_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human lymph node
Immunohistochemical staining of human lymph node shows moderate cytoplasmic positivity in non germinal center cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57179/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line GAMG
Immunofluorescent staining of human cell line GAMG shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57179/2058_E8_1_blue_red_green.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57179/131156_A_7_5.jpg
Normal Tissue
Figure 5 Testis
Sertoli cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57179/131156_A_6_6.jpg
Normal Tissue
Figure 6 Lymph node
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57179/131156_A_8_8.jpg
RNA Expression
Figure 7 RNA cell line category: Group enriched (BJ hTERT+, GAMG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000169429-CXCL8
❮
❯
Specifications
- Immunogen
- Recombinant human CXCL8 protein
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human CXCL8
- Species Reactivity
- Human
- Clone
- 4F8
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.09% sodium azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- ELISA: 1/1000
Target
- Alternative Names
- C-X-C Motif Chemokine Ligand 8; Monocyte-Derived Neutrophil Chemotactic Factor; Monocyte-Derived Neutrophil-Activating Peptide; Granulocyte Chemotactic Protein 1; Chemokine (C-X-C Motif) Ligand 8; Beta Endothelial Cell-Derived Neutrophil Activating Peptide; Lung Giant Cell Carcinoma-Derived Chemotactic Protein; Lymphocyte Derived Neutrophil Activating Peptide; Alveolar Macrophage Chemotactic Factor I; Tumor Necrosis Factor-Induced Gene 1; Neutrophil-Activating Peptide 1; T-Cell Chemotactic Factor; Interleukin 8; Emoctakin; GCP-1; MDNCF; MONAP
- Gene ID
- 3576
- UniProt ID
- P10145
- Long Name
- C-X-C Motif Chemokine Ligand 8
- Sequence Similarities
- Belongs to the intercrine alpha (chemokine CxC) family.
- Cellular Localization
- Secreted
- Post Translation Modifications
- Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.
- Protein Refseq
- NP_000575.1
- Function
- Chemotactic factor that mediates inflammatory response by attracting neutrophils, basophils, and T-cells to clear pathogens and protect the host from infection.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-WK1670. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Detection antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "CXCL8"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-738-sdAb | Recombinant Anti-human CXCL8 VHH Single Domain Antibody | IHC, ICC, FC, FA, FUNC | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-201CL | Human Anti-CXCL8 Recombinant Antibody (TAB-201CL) | ELISA | Human IgG2 |
| TAB-701LC | Human Anti-CXCL8 Recombinant Antibody (TAB-701LC) | ELISA, FC, FuncS | Human IgG1, κ |
| TAB-701LC-S(P) | Human Anti-CXCL8 Recombinant Antibody; scFv Fragment (TAB-701LC-S(P)) | ELISA | Human scFv |
| TAB-701LC-F(E) | Human Anti-CXCL8 Recombinant Antibody; Fab Fragment (TAB-701LC-F(E)) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-095LC | Recombinant Anti-Human CXCL8 Antibody (Fc glycosylation) | ELISA | Humanized antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-164LC | Recombinant Anti-Human CXCL8 Antibody (Non-glycosylated) | ELISA | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-644CQ | Mouse Anti-CXCL8 Recombinant Antibody (clone CBL601) | ELISA, Neut | Mouse IgG1 |
| NEUT-645CQ | Mouse Anti-CXCL8 Recombinant Antibody (clone CBL602) | WB, ICC, Neut | Mouse IgG1 |
| NEUT-646CQ | Mouse Anti-CXCL8 Recombinant Antibody (clone CBL115) | FC, WB, IHC, Neut | Mouse IgG1 |
| NEUT-647CQ | Mouse Anti-CXCL8 Recombinant Antibody (clone I8-60) | ELISA, Neut, WB | Mouse IgG1, κ |
| NEUT-648CQ | Mouse Anti-CXCL8 Recombinant Antibody (clone I8-S2) | ELISA, Neut, WB | Mouse IgG2b, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-660CQ | Mouse Anti-CXCL8 Recombinant Antibody (clone B-K8) | Block, ICFC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4128 | Hi-Affi™ Rabbit Anti-CXCL8 Recombinant Antibody (clone SI199DS) | ELISA | Rabbit IgG |
| MOR-4624 | Hi-Affi™ Rabbit Anti-CXCL8 Recombinant Antibody (clone TH137DS) | WB | Rabbit IgG |
| MOR-4625 | Hi-Affi™ Rabbit Anti-CXCL8 Recombinant Antibody (clone TH138DS) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-N0059-YC | Human Anti-CXCL8 Recombinant Antibody (HPAB-N0059-YC) | FuncS | Human IgG |
| HPAB-1501-FY | Mouse Anti-CXCL8 Recombinant Antibody (clone 5D2.2D10) | ELISA, WB | Mouse IgG1 |
| HPAB-1502-FY | Mouse Anti-CXCL8 Recombinant Antibody (clone 1A1.4C6) | ELISA, WB | Mouse IgG1 |
| HPAB-1503-FY | Mouse Anti-CXCL8 Recombinant Antibody (clone 1L351.17) | ELISA, WB | Mouse IgG2b |
| HPAB-A1375-YJ | Human Anti-CXCL8 Recombinant Antibody (clone K4.2) | ELISA, SPR | Human IgG2 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-N0059-YC-F(E) | Human Anti-CXCL8 Recombinant Antibody; Fab Fragment (HPAB-N0059-YC-F(E)) | FuncS | Human Fab |
| HPAB-0664-YJ-F(E) | Mouse Anti-CXCL8 Recombinant Antibody (clone 5.12.14); Fab Fragment | ELISA, WB, Inhib, Antagonist | Mouse Fab |
| HPAB-0665-YJ-F(E) | Human Anti-CXCL8 Recombinant Antibody (clone ch5.12.14); Fab Fragment | ELISA, WB, Inhib, Antagonist | Chimeric (mouse/human) Fab |
| HPAB-1841-FY-S(P) | Human Anti-CXCL8 Recombinant Antibody (clone WS-4); Fab Fragment | Inhib | Humanized Fab |
| HPAB-S0084-YC-F(E) | Mouse Anti-CXCL8 Recombinant Antibody (clone 6G4.2.5); Fab Fragment | ELISA, Inhib | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1101-FY-F(E) | Mouse Anti-CXCL8 Recombinant Antibody (clone 656.35); scFv Fragment | ELISA, IA, Block | Mouse scFv |
| HPAB-1102-FY-F(E) | Mouse Anti-CXCL8 Recombinant Antibody (clone 197.2); scFv Fragment | ELISA, IA, Block | Mouse scFv |
| HPAB-1103-FY-F(E) | Mouse Anti-CXCL8 Recombinant Antibody (clone 81.1); scFv Fragment | ELISA, IA, Block | Mouse scFv |
| HPAB-1104-FY-F(E) | Mouse Anti-CXCL8 Recombinant Antibody; Fab Fragment (HPAB-1104-FY-F(E)) | ELISA | Mouse Fab |
| HPAB-1682-FY-F(E) | Human Anti-CXCL8 Recombinant Antibody; Fab Fragment (HPAB-1682-FY-F(E)) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY593 | Anti-CXCL8 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-YC86 | Recombinant Anti-CXCL8 Eliminating Antibody, pH-Sensitive (K2.2 var.) | Antigen-Sweeping In Vivo. | Human lgG2 |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: WB, IP, IF, FuncS, FC, Neut, ELISA
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: WB, ELISA, FC, IP, FuncS, IF, Neut
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: Neut, FC, IHC-Fr, IP, BA
Application: ELISA, Inhib, FuncS
-3.jpg)
Application: IB, FC, ELISA, FuncS
Application: ELISA, FC, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
