Recombinant Mouse Anti-DES Antibody (clone 1A2-8B10-5G9)
CAT#: VS3-FY450
This product is a recombinant mouse antibody that recognizes human DES protein. The mAb was purified by affinity chromatography.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18803/1161_A10_3_selected.jpg
Normal Tissue
Figure 2 High expression in skeletal muscle
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18803/43421_B_1_6_val_selected.jpg
Normal Tissue
Figure 3 Low expression in skin 1
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18803/43421_B_7_1_val_selected.jpg
Normal Tissue
Figure 4 High expression in heart muscle.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/34/1988_B_5_6_selected.jpg
Normal Tissue
Figure 5 Skeletal muscle
Myocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18803/43421_B_1_6.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (HSkMC, LHCN-M2, NB-4, RH-30)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000175084-DES
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IHC
Figure 1. Recombinant Mouse Anti-DES Antibody (clone 1A2-8B10-5G9) in IHC.
Immunohistochemical analysis of paraffin-embedded appendix using Desmin antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Specifications
- Immunogen
- Synthesized peptide derived from human Desmin
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human DES
- Species Reactivity
- Human
- Clone
- 1A2-8B10-5G9
- Applications
- Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity Chromatography Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- PBS pH 7.3, 50% Glycerol, 0.5% BSA
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry: 1/100-1/500
Target
- Alternative Names
- Desmin; Intermediate Filament Protein; Cardiomyopathy, Dilated 1F (Autosomal Dominant); Mutant Desmin P.K241E; LGMD1D; LGMD1E
- Gene ID
- 1674
- UniProt ID
- P17661
- Sequence Similarities
- Belongs to the intermediate filament family.
- Cellular Localization
- Cell membrane, Cytoplasm, Intermediate filament, Membrane, Nucleus
- Post Translation Modifications
- ADP-ribosylation prevents ability to form intermediate filaments.
Phosphorylation at Ser-7, Ser-28 and Ser-32 by CDK1, phosphorylation at Ser-60 by AURKB and phosphorylation at Thr-76 by ROCK1 contribute to efficient separation of desmin intermediate filaments during mitosis.
Ubiquitination by a SCF-like complex containing ASB2 isoform 1 leads to proteasomal degradation.
- Protein Refseq
- NP_001918.3
- Function
- Muscle-specific type III intermediate filament essential for proper muscular structure and function. Plays a crucial role in maintaining the structure of sarcomeres, inter-connecting the Z-disks and forming the myofibrils, linking them not only to the sarcolemmal cytoskeleton, but also to the nucleus and mitochondria, thus providing strength for the muscle fiber during activity (PubMed:25358400).
In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures (PubMed:24200904, PubMed:25394388, PubMed:26724190).
May act as a sarcomeric microtubule-anchoring protein: specifically associates with detyrosinated tubulin-alpha chains, leading to buckled microtubules and mechanical resistance to contraction. Contributes to the transcriptional regulation of the NKX2-5 gene in cardiac progenitor cells during a short period of cardiomyogenesis and in cardiac side population stem cells in the adult. Plays a role in maintaining an optimal conformation of nebulette (NEB) on heart muscle sarcomeres to bind and recruit cardiac alpha-actin (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "DES"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1487z | Mouse Anti-DES Recombinant Antibody (clone 25C5) | WB, ELISA, IHC | Mouse IgG1 |
| MOB-1093MZ | Recombinant Mouse Anti-Human DES Antibody (clone E44) | ICC, IF, IHC-Fr IHC-P, WB | Mouse antibody |
| ZG-0001C | Mouse Anti-DES Recombinant Antibody (clone ABT-DES) | IHC-P, IF | Mouse IgG |
| ZG-1018F | Mouse Anti-DES Recombinant Antibody (clone D33) | ICC, IHC-Fr, IHC-P, WB | Mouse IgG1 |
| ZG-1019F | Mouse Anti-DES Recombinant Antibody (clone D9) | IHC-Fr, IHC-P, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0971 | Hi-Affi™ Rabbit Anti-DES Recombinant Antibody (clone DS971AB) | WB, IHC-P | Rabbit IgG |
| MOR-0087-FY | Rabbit Anti-DES Recombinant Antibody (clone AFY0058) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX325 | Mouse Anti-DES Recombinant Antibody (clone 290CT7.1.1) | WB | Mouse IgM, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY161 | Mouse Anti-NHP DES Recombinant Antibody (clone D33) | WB, IHC, IF | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC298 | CytoStream™ Rabbit Anti-DES Recombinant Antibody (VS13-YC298) | WB, ICC, IF, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1976 | Anti-DES Immunohistochemistry Kit | IHC | |
| VS-0525-XY1977 | Anti-Mouse DES Immunohistochemistry Kit | IHC | |
| VS-0525-XY1978 | Anti-Rat DES Immunohistochemistry Kit | IHC |
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