Recombinant Rabbit Anti-EIF4E Antibody (clone R04-4H7)
CAT#: VS3-FY489
This product is a recombinant rabbit antibody that recognizes human, mouse and rat EIF4E protein. It can detect a band of approximately 25 kDa (observedd molecular weight: 25 kDa).
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & cytoplasmic bodies.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68358/1607_H9_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16316/951_E2_2_selected.jpg
Normal Tissue
Figure 3 Testis
Elongated or late spermatids Staining: High Intensity: Strong Quantity:>75% Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4077/11592_A_6_6.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4077/11592_A_9_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4077/11592_A_8_5.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000151247-EIF4E
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WB
Figure 1. Recombinant Rabbit Anti-EIF4E Antibody (clone R04-4H7) in WB.
Western blot analysis of eIF4E in K562, rat brain, C6, 3T3, Hela lysates using eIF4E Antibody.
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Specifications
- Immunogen
- A synthetic peptide of human eIF4E
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat EIF4E
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R04-4H7
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 25 kDa; Observed MW: 25 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- Eukaryotic Translation Initiation Factor 4E; MRNA Cap-Binding Protein; EIF-4F 25 KDa Subunit; EIF4EL1; EIF-4E; EIF4F
- Gene ID
- 1977
- UniProt ID
- P06730
- Sequence Similarities
- Belongs to the eukaryotic initiation factor 4E family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.
- Protein Refseq
- NP_001124150.1; NP_001124151.1; NP_001317946.1
- Function
- Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
Component of a multiprotein complex that sequesters and represses translation of proneurogenic factors during neurogenesis (By similarity).
In P-bodies, component of a complex that mediates the storage of translationally inactive mRNAs in the cytoplasm and prevents their degradation (PubMed:24335285).
May play an important role in spermatogenesis through translational regulation of stage-specific mRNAs during germ cell development (By similarity).
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Related Signaling Pathways
Insulin Signaling Pathway
MAPK Signaling Pathway
mTOR Signaling Pathway
Related Diseases
Insulin Resistance
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1128 | Hi-Affi™ Rabbit Anti-EIF4E Recombinant Antibody (clone DS1128AB) | WB, IP, IHC-P, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0648J | Rabbit Anti-EIF4E Recombinant Antibody (clone 1F3) | ELISA, WB, IHC | Rabbit IgG |
| VS3-FY490 | Recombinant Rabbit Anti-EIF4E Antibody (clone R04-5A4) | WB, IHC-F, IHC-P, ICC, IF, IP | Rabbit IgG |
| VS3-FY491 | Recombinant Rabbit Anti-EIF4E Antibody (clone R08-1C5) | WB, ICC, IF, IP | Rabbit IgG |
| VS7-HM850 | Mouse Anti-EIF4E Recombinant Antibody (clone 5D11) | WB, IHC, ICC, FCM | Mouse IgG1 |
| VS7-HM851 | Mouse Anti-EIF4E Recombinant Antibody (clone CBL221HM) | WB, IHC-p, IF, ICC, FCM, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY752 | Anti-EIF4E Immunohistochemistry Kit | IHC | |
| VS-0525-XY2229 | Anti-Mouse EIF4E Immunohistochemistry Kit | IHC | |
| VS-0525-XY2228 | Anti-Human EIF4E Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC350 | CytoStream™ Rabbit Anti-EIF4E Recombinant Antibody (VS13-YC350) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
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