Recombinant Rabbit Anti-FBL Antibody (clone R09-7E5)
CAT#: VS3-FY536
This product is a recombinant rabbit antibody that recognizes FBL. This antibody has been reported for use in Western Blot, Immunoprecipitation. The clone R09-7E5 is specific for human, mouse, rat FBL. The antigen is synthetic peptide of human fibrin.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nucleoli fibrillar center.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49546/756_G8_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli fibrillar center.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1514/652_H1_1_selected.jpg
Normal Tissue
Figure 3 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows nuclear positivity in exocrine glandular cells and cells in islets of Langerhans.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/77724/168552_A_2_3_selected.jpg
Normal Tissue
Figure 4 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows strong nuclear positivity in purkinje cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1514/ihc_selected.jpg
Normal Tissue
Figure 5 Cerebral cortex
Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: nuclear Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1514/4603_B_9_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1514/4603_A_8_3.jpg
Normal Tissue
Figure 7 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1514/4603_A_6_6.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000105202-FBL
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WB
Figure 1. Recombinant Rabbit Anti-FBL Antibody (clone R09-7E5) in WB.
Western Blot analysis of fibrillin in K562, C6, 3T3 lysates using fibrillin antibody.
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Specifications
- Immunogen
- Synthetic peptide of human fibrin.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat FBL
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R09-7E5
- Applications
- Western Blot, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 34 kDa; Observed MW: 34 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "FBL"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1573z | Mouse Anti-FBL Recombinant Antibody (clone 31A4) | WB, ELISA, IP | Mouse IgG1 |
| MRO-1863-CN | Rabbit Anti-FBL Polyclonal Antibody (MRO-1863-CN) | WB, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1255 | Hi-Affi™ Rabbit Anti-FBL Recombinant Antibody (clone DS1255AB) | WB, IHC-P, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC3375 | PE-A1/Human FBL (VSDIVGPDGLVY) MHC Tetramer | FCM | |
| MHC-LC3406 | APC-A1/Human FBL (VSDIVGPDGLVY) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC408 | AbPlus™ Anti-FBL Magnetic Beads (VS-0724-YC408) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY203 | Rabbit Anti-NHP FBL Recombinant Antibody (VS-1024-XY203) | WB, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY818 | Anti-FBL Immunohistochemistry Kit | IHC | |
| VS-0525-XY2456 | Anti-Mouse FBL Immunohistochemistry Kit | IHC | |
| VS-0525-XY2455 | Anti-Human FBL Immunohistochemistry Kit | IHC |
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