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Recombinant Rabbit Anti-FH Antibody (clone R04-5B6)

Recombinant Rabbit Anti-FH Antibody (clone R04-5B6) (CAT#: VS3-FY550)

Name: Recombinant Rabbit Anti-FH Antibody (clone R04-5B6)
SKU: VS3-FY550
Rating: 5 (1 reviews)

This product is a recombinant rabbit antibody that recognizes FH. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The clone R04-5B6 is specific for human, mouse, rat, Hamster FH. The antigen is human fh recombinant protein.

Custom Production

We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

We require custom production

Tested Data
Gene Expression
Datasheet
MSDS
COA

Figure 1. Recombinant Rabbit Anti-FH Antibody (clone R04-5B6) in WB.

Figure 1. Recombinant Rabbit Anti-FH Antibody (clone R04-5B6) in WB.

Western Blot analysis of FH/Fumarase in Jurkat, Rat Brain, C6, CHO-K1 lysates using FH/Fumarase Antibody.

Subcellular Location

Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17785/if_selected.jpg

Normal Tissue

Figure 2 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27341/58998_B_8_5.jpg

Figure 3 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27341/58998_A_8_3.jpg

Figure 4 Kidney
(Bowman's capsule Staining: Medium Intensity: Moderate Quantity:>75% Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Collecting ducts Staining: High Intensity: Strong Quantity:>75% Distal tubules Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27341/58998_A_8_5.jpg

Figure 5 Testis
(Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27341/58998_A_4_6.jpg

Figure 6 Skin
(Epidermal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27341/58998_B_4_8.jpg

Figure 7 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27341/58998_A_8_8.jpg

RNA Expression

Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000091483-FH

Datasheet

MSDS

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
Human FH recombinant protein.

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human, Mouse, Rat, Hamster FH

Species Reactivity
Human, Mouse, Rat, Hamster

Clone
R04-5B6

Applications
Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation

Conjugate
Unconjugated

MW
Calculated MW: 55 kDa; Observed MW: 49 kDa

Product Property

Purification
Affinity Purified

Purity
>95% as determined by SDS-PAGE

Buffer
50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA

Preservative
0.01% Sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20

Target

Alternative Names
Fumarate Hydratase; EC 4.2.1.2; Fumarase; Fumarate Hydratase, Mitochondrial; HLRCC; MCUL1; FMRD; LRCC; MCL

Gene ID
2271

UniProt ID
P07954

Cellular Localization
Chromosome, Mitochondrion, Cytoplasm, Nucleus

Secondary Antibody

Isotype Control

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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IgG Antibody Products Antibody Magnetic Beads IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry

CAT	Product Name	Application	Type
ZG-0033C	Mouse Anti-FH Recombinant Antibody (clone 2B11)	WB, IF/ICC	Mouse IgG
ZG-0034C	Mouse Anti-FH Recombinant Antibody (clone 7F1)	WB, IHC-P, IF/ICC	Mouse IgG
VS3-CJ51	Mouse Anti-FH Recombinant Antibody (VS3-CJ51)	WB, ELISA	Mouse IgG
VS3-CJ480	Mouse Anti-FH Recombinant Antibody (VS3-CJ480)	WB, ICC, IF	Mouse IgG
VS3-CJ481	Mouse Anti-FH Recombinant Antibody (VS3-CJ481)	WB, IHC	Mouse IgG

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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