Recombinant Rabbit Anti-GRIA1 (phospho Ser845) Antibody (clone R03-7G4) (CAT#: VS3-FY614)

• Immunogen
• A synthetic phosphopeptide corresponding to residues surrounding Ser845 of human AMPA receptor 1 (GluA1).

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Rat GRIA1

• Species Reactivity
• Human, Rat

• Clone
• R03-7G4

• Applications
• Western Blot

• Conjugate
• Unconjugated

• MW
• Calculated MW: 102 kDa; Observed MW: 102 kDa

• Purification
• Affinity Purified

• Purity
• >95% as determined by SDS-PAGE

• Buffer
• 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA

• Preservative
• 0.01% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000

• Alternative Names
• Glutamate Ionotropic Receptor AMPA Type Subunit 1; Glutamate Receptor, Ionotropic, AMPA 1; AMPA-Selective Glutamate Receptor 1; GluR-K1; GluR-1; GluR-A; GLUR1; GLUH1

• Gene ID
• 2890

• UniProt ID
• P42261

• Sequence Similarities
• Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily.

• Cellular Localization
• Cell membrane, Cell projection, Endoplasmic reticulum, Endosome, Membrane, Postsynaptic cell membrane, Synapse

• Post Translation Modifications
• Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis (By similarity).
  Phosphorylated at Ser-645. Phosphorylated at Ser-710 by PKC. Phosphorylated at Ser-849 by PKC, PKA and CAMK2. Phosphorylated at Ser-863 by PKC, PKA and PRKG2 (By similarity).
  Phosphorylation of Ser-863 is reduced by induction of long-term depression and increased by induction of long-term potentiation (By similarity).

• Protein Refseq
• NP_000818.2; NP_001107655.1; NP_001244948.1

• Function
• Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody

• 2ND-057CQ
• Donkey Anti-Rabbit IgG (H&L) Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-060CQ
• Llama Anti-Rabbit IgG (H&L) Antibody

• 2ND-061CQ
• Sheep Anti-Rabbit IgG (H&L) Antibody

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody