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    Recombinant Rabbit Anti-HSPA1A Antibody (clone R04-7A2) (CAT#: VS3-FY707)

    This product is a recombinant rabbit antibody that recognizes HSPA1A. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence. The clone R04-7A2 is specific for human, mouse, rat HSPA1A. The antigen is synthetic peptide of human hsp70.

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
    Modalities :
    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line CACO-2
    Figure 1 IF staining of human cell line CACO-2
    (Immunofluorescent staining of human cell line CACO-2 shows localization to vesicles.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/52504/1028_G12_5_selected.jpg
    Figure 2 IF staining of human cell line A-431
    Figure 2 IF staining of human cell line A-431
    (Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/32815/if_selected.jpg
    Normal Tissue
    Figure 3 IHC staining of human testis
    Figure 3 IHC staining of human testis
    (Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in subset of cells in seminiferus ducts.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/52504/ihc_selected.jpg
    Figure 4 IHC staining of human prostate
    Figure 4 IHC staining of human prostate
    (Immunohistochemical staining of human prostate shows strong cytoplasmic and nuclear positivity in the basal layer of glandular cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/17451/71979_A_1_5_selected.jpg
    Figure 5 Cerebral cortex
    Figure 5 Cerebral cortex
    (Neuronal cells
    Staining: Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/17451/71979_B_7_5.jpg
    Figure 6 Colon
    Figure 6 Colon
    (Endothelial cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear
    Glandular cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/17451/71979_A_7_3.jpg
    Figure 7 Liver
    Figure 7 Liver
    (Cholangiocytes
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear
    Hepatocytes
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/17451/71979_A_9_4.jpg
    Figure 8 Kidney
    Figure 8 Kidney
    (Cells in tubules
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/17451/71979_A_9_5.jpg
    Figure 9 Testis
    Figure 9 Testis
    (Cells in seminiferous ducts
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear
    Leydig cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/32815/72080_A_6_6.jpg
    Figure 10 Lymph node
    Figure 10 Lymph node
    (Germinal center cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear
    Non-germinal center cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/32815/72080_A_9_8.jpg
    RNA Expression
    Figure 11 RNA cell line category: Cell line enriched (HEK93)
    Figure 11 RNA cell line category: Cell line enriched (HEK93)
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000204389-HSPA1A

    Specifications

    • Immunogen
    • Synthetic peptide of human Hsp70.
    • Host Species
    • Rabbit
    • Type
    • Rabbit IgG
    • Specificity
    • Human, Mouse, Rat HSPA1A
    • Species Reactivity
    • Human, Mouse, Rat
    • Clone
    • R04-7A2
    • Applications
    • Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence
    • Conjugate
    • Unconjugated
    • MW
    • Calculated MW: 70 kDa; Observed MW: 70 kDa

    Product Property

    • Purification
    • Affinity Purified
    • Purity
    • >95% as determined by SDS-PAGE
    • Buffer
    • 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
    • Preservative
    • 0.01% Sodium azide
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

    Applications

    • Application Notes
    • Western Blot: 1/500-1/1000
      Immunohistochemistry-Paraffin: 1/50-1/100
      Immunofluorescence: 1/50-1/200

    Target

    • Alternative Names
    • Heat Shock Protein Family A (Hsp70) Member 1A; Heat Shock 70kDa Protein 1A; Heat Shock 70 KDa Protein 1; Heat Shock 70kD Protein 1A; HSP70-1; HSP70.1; HSPA1; HSP72; DnaK-Type Molecular Chaperone HSP70-1; Epididymis Secretory Protein Li 103; Heat Shock 70 KDa Protein 1A/1B
    • Cellular Localization
    • Cytoplasm, Cytoskeleton, Nucleus, Secreted

    Recommended Products

    Secondary Antibody

    Isotype Control

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "HSPA1A"

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    Fab Antibodies ScFv Antibodies IgG Antibodies IgM Antibodies Blocking Antibodies Rabbit Monoclonal Antibodies Antibody Magnetic Beads ScFv-Fc Chimeras IHC Kits & Antibodies
    CAT Product Name Application Type
    MOB-517-F(E) Recombinant Anti-human HSPA1A Antibody Fab Fragment WB, RIA, FuncS Fab
    MHH-517-F(E) Recombinant Human Anti-human HSPA1A Antibody Fab Fragment WB, FC, FuncS Fab
    HPAB-0243-YC-F(E) Mouse Anti-HSPA1A Recombinant Antibody Fab Fragment (HPAB-0243-YC-F(E)) ELISA Mouse Fab
    HPAB-0244-YC-F(E) Human Anti-HSPA1A Recombinant Antibody Fab Fragment (HPAB-0244-YC-F(E)) ELISA Humanized Fab

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