Recombinant Mouse Anti-IL18 Antibody (VS-0923-FY191)
CAT#: VS-0923-FY191
The VS-0923-FY191 is a murine IgG2b antibody that binds to IL18. This antibody was originally generated in mice immuned with the full length of human IL18.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3980/16_G12_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3980/14_G12_1_red_green.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3980/13_G12_1_red_green.jpg
Normal Tissue
Figure 4 Colon
Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7772/19579_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7772/19579_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Collecting ducts
Staining: Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining: Medium
Intensity: Strong
Quantity: <25%
Proximal tubules (microvilli)
Staining: Medium
Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7772/19579_A_9_5.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7772/19579_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (A-431, CAPAN-2, HaCaT, HBEC3-KT, hTCEpi, PC-3, U-2 OS)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000150782-IL18
❮
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Specifications
- Immunogen
- The full-length IL-18 protein
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human IL18
- Species Reactivity
- Human
- Applications
- ELISA, Western Blot, Immunohistochemistry
- Conjugate
- Unconjugated
Product Property
- Format
- Liquid
- Buffer
- Citrate Tris HCl buffer
- Preservative
- 0.02% Proclin 300
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot (1/1000)
Immunohistochemistry (1/10)
Specific conditions should be optimized by the end user.
Target
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Related Diseases
Inflammatory Bowel Diseases
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "IL18"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0373CL | Human Anti-IL18 Recombinant Antibody (TAB-0373CL) | ELISA, FuncS, Inhib | Human IgG |
| TAB-0374CL | Human Anti-IL18 Recombinant Antibody (TAB-0374CL) | ELISA, FuncS, Inhib | Human IgG |
| TAB-0373CL-S(P) | Human Anti-IL18 Recombinant Antibody; scFv Fragment (TAB-0373CL-S(P)) | ELISA, FuncS, Inhib | Human scFv |
| TAB-0374CL-S(P) | Human Anti-IL18 Recombinant Antibody; scFv Fragment (TAB-0374CL-S(P)) | ELISA, FuncS, Inhib | Human scFv |
| TAB-0373CL-F(E) | Human Anti-IL18 Recombinant Antibody; Fab Fragment (TAB-0373CL-F(E)) | ELISA, FuncS, Inhib | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0290MZ | Chicken Anti-Interleukin-18 Polyclonal IgY | Indirect ELISA, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1238CQ | Rabbit Anti-IL18 Recombinant Antibody (NEUT-1238CQ) | Neut | Rabbit IgG |
| NEUT-1239CQ | Rat Anti-Il18 Recombinant Antibody (clone 39-3F) | Neut, WB | Rat IgG2a |
| NEUT-1240CQ | Mouse Anti-IL18 Recombinant Antibody (clone CBL670) | Neut | Mouse IgG2a |
| NEUT-1241CQ | Rat Anti-Il18 Recombinant Antibody (clone YIGIF74-1G7) | Neut | Rat IgG2a |
| NEUT-1242CQ | Rat Anti-Il18 Recombinant Antibody (clone 93-10C) | IP, Neut | Rat IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4248 | Rabbit Anti-IL18 Recombinant Antibody (clone SI327DS) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0066-YC | Human Anti-IL18 Recombinant Antibody (HPAB-0066-YC) | Neut | Humanized IgG2 |
| HPAB-0067-YC | Rat Anti-IL18 Recombinant Antibody (clone 2C10) | Neut | Rat IgG |
| HPAB-0064-YC | Human Anti-IL18 Recombinant Antibody (clone 2E1) | ELISA, RIA, IHC, FuncS, Neut | Human IgG |
| HPAB-0649-CN | Human Anti-IL18 Recombinant Antibody (HPAB-0649-CN) | ELISA, Neut | Humanized IgG1, κ |
| FAMAB-0041JF | Human Anti-IL18 Recombinant Antibody (clone h18-108) | WB, IF, IHC, ELISA | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0065-YC-F(E) | Human Anti-IL18 Recombinant Antibody (clone LT28); Fab Fragment | ELISA, RIA, IHC, FuncS, Neut | Human Fab |
| HPAB-0855-FY-S(P) | Human Anti-IL18 Recombinant Antibody (clone 2.5); Fab Fragment | ELISA | Human Fab |
| HPAB-0856-FY-S(P) | Human Anti-IL18 Recombinant Antibody; scFv Fragment (HPAB-0856-FY-S(P)) | Inhib | Human scFv |
| HPAB-0857-FY-S(P) | Human Anti-IL18 Recombinant Antibody; scFv Fragment (HPAB-0857-FY-S(P)) | Inhib | Human scFv |
| HPAB-1558-FY-S(P) | Human Anti-IL18 Recombinant Antibody (clone H1L2); Fab Fragment | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0229-CN-S(P) | Human Anti-IL18 Recombinant Antibody (clone 1I1G); scFv Fragment | WB, ELISA | Human scFv |
| FAMAB-0230-CN-S(P) | Human Anti-IL18 Recombinant Antibody (clone 3I1E); scFv Fragment | WB, ELISA | Human scFv |
| FAMAB-0231-CN-S(P) | Human Anti-IL18 Recombinant Antibody (clone 4I3E); scFv Fragment | WB, ELISA | Human scFv |
| FAMAB-0232-CN-S(P) | Human Anti-IL18 Recombinant Antibody (clone 5I4E); scFv Fragment | WB, ELISA | Human scFv |
| HPAB-1558-FY-F(E) | Human Anti-IL18 Recombinant Antibody (clone H1L2); scFv Fragment | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1118 | AbPlus™ Anti-IL18 Magnetic Beads (VS-0724-YC1118) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0125-FY16 | Human Anti-IL18 (clone ABT-325) scFv-Fc Chimera | ELISA | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1117 | Anti-IL18 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3500 | Anti-Human IL18 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC111 | Recombinant Anti-IL18 (AA 37-50 x AA 64-80) Biparatopic Antibody, Tandem scFv (Clone LT28 x Clone 2E1) | ELISA, IHC, Neut | Tandem scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-YC170 | Recombinant Anti-IL18 Eliminating Antibody, pH-Sensitive (2E1 var.) | Antigen-Sweeping In Vivo. | Human IgG |
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