Recombinant Anti-IL18 (AA 37-50 x AA 64-80) Biparatopic Antibody, Tandem scFv (Clone LT28 x Clone 2E1)
CAT#: VS-0525-YC111
The Recombinant Anti-IL18 (AA 37-50 x AA 64-80) Biparatopic Antibody, Tandem scFv (Clone LT28 x Clone 2E1) functions as a biparatopic antibody (bpAb), engaging two distinct IL18 epitopes. Its tandem scFv design positions the LT28 scFv (targeting amino acids 37-50, sequence: YFGKLESKLSVIRN) N-terminally to the 2E1 scFv (specific for amino acids 64-80, sequence: PLFEDMTDSDCR).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3980/16_G12_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3980/14_G12_1_red_green.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3980/13_G12_1_red_green.jpg
Normal Tissue
Figure 4 Colon
Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7772/19579_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7772/19579_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Collecting ducts
Staining: Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining: Medium
Intensity: Strong
Quantity: <25%
Proximal tubules (microvilli)
Staining: Medium
Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7772/19579_A_9_5.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7772/19579_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (A-431, CAPAN-2, HaCaT, HBEC3-KT, hTCEpi, PC-3, U-2 OS)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000150782-IL18
❮
❯
Specifications
- Host Animal 1
- Human
- Host Animal 2
- Human
- Specificity
- Human IL18
- Species Reactivity
- Human
- Type
- Tandem scFv
- Valency
- 1 + 1
- Clone 1
- LT28
- Clone 2
- 2E1
- Epitope 1
- aa 37-50, YFGKLESKLSVIRN
- Epitope 2
- aa 64-80, PLFEDMTDSDCR
- Purity
- >90%
- Purification
- Affinity purified
- Applications
- ELISA, IHC, Neut
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid freeze-thaw cycles.
- Long Name
- Interleukin 18
Target
- Introduction
- Interleukin-18 (IL-18, also known as interferon-gamma inducing factor) is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of IL-18 and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. IL-18 is an IL-1−like cytokine that requires cleavage with caspase-1 to become active, was found to increase IgE production in a CD4+ T cells-, IL-4− and STAT6−dependent fashion. IL-18 and T cell receptor−mediated stimulation could induce naïve CD4+ T cells to develop into IL-4−producing cells in vitro. Thus, caspase-1 and IL-18 may be critical in regulation of IgE production in vivo, providing a potential therapeutic target for allergic disorders. IL-18 production in primary synovial cultures and purified synovial fibroblasts was, in turn, upregulated by TNF-α and IL-1β, suggesting that monokine expression can feed back to promote Th1 cell development in synovial membrane. Besides, synergistic combinations of IL-18, IL-12, and IL-15 may be of importance in sustaining both Th1 responses and monokine production in RA.
- Alternative Names
- IGIF; IL-18; IL-1g; IL1F4
- Gene ID
- 3606
- UniProt ID
- Q14116
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Related Diseases
Inflammatory Bowel Diseases
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Datasheet
MSDS
COA
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Protocol & Troubleshooting
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|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBW-081 | Mouse Anti-IL18 Recombinant Antibody (clone 125-2H); Fab Fragment | ELISA, Neut | Mouse Fab |
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| HPAB-0857-FY-S(P) | Human Anti-IL18 Recombinant Antibody; scFv Fragment (HPAB-0857-FY-S(P)) | Inhib | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBW-081 | Mouse Anti-IL18 Recombinant Antibody (clone 125-2H); scFv Fragment | ELISA, Neut | Mouse scFv |
| HPAB-0649-CN-S(P) | Human Anti-IL18 Recombinant Antibody; scFv Fragment (HPAB-0649-CN-S(P)) | ELISA, Neut | Humanized scFv |
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| HPAB-1273LY-S(P) | Human Anti-IL18 Recombinant Antibody; scFv Fragment (HPAB-1273LY-S(P)) | ELISA, Inhib | Human scFv |
| HPAB-1274LY-S(P) | Human Anti-IL18 Recombinant Antibody; scFv Fragment (HPAB-1274LY-S(P)) | ELISA, Inhib | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0373CL | Human Anti-IL18 Recombinant Antibody (TAB-0373CL) | ELISA, FuncS, Inhib | Human IgG |
| TAB-0374CL | Human Anti-IL18 Recombinant Antibody (TAB-0374CL) | ELISA, FuncS, Inhib | Human IgG |
| TAB-0373CL-S(P) | Human Anti-IL18 Recombinant Antibody; scFv Fragment (TAB-0373CL-S(P)) | ELISA, FuncS, Inhib | Human scFv |
| TAB-0374CL-S(P) | Human Anti-IL18 Recombinant Antibody; scFv Fragment (TAB-0374CL-S(P)) | ELISA, FuncS, Inhib | Human scFv |
| TAB-0373CL-F(E) | Human Anti-IL18 Recombinant Antibody; Fab Fragment (TAB-0373CL-F(E)) | ELISA, FuncS, Inhib | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0290MZ | Chicken Anti-Interleukin-18 Polyclonal IgY | Indirect ELISA, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| NEUT-1239CQ | Rat Anti-Il18 Recombinant Antibody (clone 39-3F) | Neut, WB | Rat IgG2a |
| NEUT-1240CQ | Mouse Anti-IL18 Recombinant Antibody (clone CBL670) | Neut | Mouse IgG2a |
| NEUT-1241CQ | Rat Anti-Il18 Recombinant Antibody (clone YIGIF74-1G7) | Neut | Rat IgG2a |
| NEUT-1242CQ | Rat Anti-Il18 Recombinant Antibody (clone 93-10C) | IP, Neut | Rat IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4248 | Rabbit Anti-IL18 Recombinant Antibody (clone SI327DS) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0125-FY16 | Human Anti-IL18 (clone ABT-325) scFv-Fc Chimera | ELISA | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1117 | Anti-IL18 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3500 | Anti-Human IL18 Immunohistochemistry Kit | IHC |
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