Human Anti-IL18 (clone ABT-325) scFv-Fc Chimera
CAT#: VS-0125-FY16
The function of the anti-hIL-18 ScFv-Fc fusion protein primarily lies in enhancing the affinity towards human interleukin-18 (hIL-18). Specifically, the ScFv-Fc fusion protein exhibits a higher binding capacity than the individual components when combining the ScFv (single-chain antibody fragment) with the Fc (crystalline domain) region. The fusion protein with a C-terminal Fc domain shows approximately fourfold greater affinity for hIL-18 compared to the standalone anti-hIL-18 ScFv. Additionally, the anti-hIL-18 ScFv-Fc may not only enhance binding affinity to hIL-18 but also play a role in modulating or enhancing signaling pathways, thereby promoting more effective therapeutic outcomes or biological functions.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3980/16_G12_1_selected.jpg
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Specifications
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Stabilizer
- No Stabilizers
- Sterility
- 0.2 μM filtered
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- The primary function of anti-hIL-18 ScFv-Fc is to enhance the affinity towards human interleukin-18 (hIL-18).
Target
- Alternative Names
- Interleukin 18
- Gene ID
- 3606
- UniProt ID
- Q14116
- Sequence Similarities
- Belongs to the IL-1 family.
- Cellular Localization
- Cytoplasm, cytosol
Secreted
- Post Translation Modifications
- The pro-IL-18 precursor is processed by CASP1, CASP4 or CASP5 to yield its mature, active form.
Modification sites at PhosphoSitePlus
Modification sites at neXtProt
Glycosylation from GlyGen 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_011541107.1; XP_054224645.1; NP_001230140.1; NP_001373349.1; NP_001553.1
- Function
- Pro-inflammatory cytokine primarily involved in epithelial barrier repair, polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses.
Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators.
Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells.
Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.
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Related Diseases
Inflammatory Bowel Diseases
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "Clone ABT-325"
- CAT
- Product Name
See other products for "IL18"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-602 | Human Anti-IL18 Recombinant Antibody (clone YC127); scFv Fragment | WB, ELISA, FuncS | Human scFv |
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| HPAB-0064-YJ-S(P) | Rat Anti-IL18 Recombinant Antibody (clone 14B7); scFv Fragment | IA, Neut | Rat scFv |
| HPAB-0070-YJ-S(P) | Human Anti-IL18 Recombinant Antibody; scFv Fragment (HPAB-0070-YJ-S(P)) | ELISA, Neut, IP, FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0373CL | Human Anti-IL18 Recombinant Antibody (TAB-0373CL) | ELISA, FuncS, Inhib | Human IgG |
| TAB-0374CL | Human Anti-IL18 Recombinant Antibody (TAB-0374CL) | ELISA, FuncS, Inhib | Human IgG |
| TAB-0373CL-S(P) | Human Anti-IL18 Recombinant Antibody; scFv Fragment (TAB-0373CL-S(P)) | ELISA, FuncS, Inhib | Human scFv |
| TAB-0373CL-F(E) | Human Anti-IL18 Recombinant Antibody; Fab Fragment (TAB-0373CL-F(E)) | ELISA, FuncS, Inhib | Human Fab |
| TAB-0374CL-F(E) | Human Anti-IL18 Recombinant Antibody; Fab Fragment (TAB-0374CL-F(E)) | ELISA, FuncS, Inhib | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1238CQ | Rabbit Anti-IL18 Recombinant Antibody (NEUT-1238CQ) | Neut | Rabbit IgG |
| NEUT-1239CQ | Rat Anti-Il18 Recombinant Antibody (clone 39-3F) | Neut, WB | Rat IgG2a |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| FAMAB-0232-CN | Human Anti-IL18 Recombinant Antibody (clone 5I4E) | WB, ELISA | Human IgG, κ |
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|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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