Human Anti-IL18 (clone ABT-325) scFv-Fc Chimera
CAT#: VS-0125-FY16
The function of the anti-hIL-18 ScFv-Fc fusion protein primarily lies in enhancing the affinity towards human interleukin-18 (hIL-18). Specifically, the ScFv-Fc fusion protein exhibits a higher binding capacity than the individual components when combining the ScFv (single-chain antibody fragment) with the Fc (crystalline domain) region. The fusion protein with a C-terminal Fc domain shows approximately fourfold greater affinity for hIL-18 compared to the standalone anti-hIL-18 ScFv. Additionally, the anti-hIL-18 ScFv-Fc may not only enhance binding affinity to hIL-18 but also play a role in modulating or enhancing signaling pathways, thereby promoting more effective therapeutic outcomes or biological functions.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3980/16_G12_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3980/14_G12_1_red_green.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3980/13_G12_1_red_green.jpg
Normal Tissue
Figure 4 Colon
Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7772/19579_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7772/19579_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Collecting ducts
Staining: Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining: Medium
Intensity: Strong
Quantity: <25%
Proximal tubules (microvilli)
Staining: Medium
Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7772/19579_A_9_5.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7772/19579_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (A-431, CAPAN-2, HaCaT, HBEC3-KT, hTCEpi, PC-3, U-2 OS)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000150782-IL18
❮
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Specifications
- Host Species
- Human
- Type
- Human IgG1, scFv-Fc
- Species Reactivity
- Human
- Clone
- ABT-325
- Applications
- ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Stabilizer
- No Stabilizers
- Sterility
- 0.2 μM filtered
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- The primary function of anti-hIL-18 ScFv-Fc is to enhance the affinity towards human interleukin-18 (hIL-18).
Target
- Alternative Names
- Interleukin 18
- Gene ID
- 3606
- UniProt ID
- Q14116
- Sequence Similarities
- Belongs to the IL-1 family.
- Cellular Localization
- Cytoplasm, cytosol
Secreted
- Post Translation Modifications
- The pro-IL-18 precursor is processed by CASP1, CASP4 or CASP5 to yield its mature, active form.
Modification sites at PhosphoSitePlus
Modification sites at neXtProt
Glycosylation from GlyGen 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_011541107.1; XP_054224645.1; NP_001230140.1; NP_001373349.1; NP_001553.1
- Function
- Pro-inflammatory cytokine primarily involved in epithelial barrier repair, polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses.
Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators.
Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells.
Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.
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Related Diseases
Inflammatory Bowel Diseases
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "Clone ABT-325"
- CAT
- Product Name
See other products for "IL18"
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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