Recombinant Rabbit Anti-ISL1 Antibody (clone R05-2C3)
CAT#: VS3-FY768
This product is a recombinant rabbit antibody that recognizes ISL1. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation. The clone R05-2C3 is specific for human, rat ISL1. The antigen is human islet 1 recombinant protein.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line SH-SY5Y
Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm & mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48613/1792_G11_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human caudate
Immunohistochemical staining of human caudate shows strong nuclear positivity in a subset of glial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/57416/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/57416/132766_B_7_5.jpg
Normal Tissue
Figure 4 Caudate
Glial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/57416/132766_B_7_7.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/57416/132766_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/57416/132766_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/57416/132766_A_5_6.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enriched (SH-SY5Y)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000016082-ISL1
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WB
Figure 1. Recombinant Rabbit Anti-ISL1 Antibody (clone R05-2C3) in WB.
Western Blot analysis of islet 1 from rat brain lysate K562 using islet 1 antibody.
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Specifications
- Immunogen
- human islet 1 recombinant protein.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Rat ISL1
- Species Reactivity
- Human, Rat
- Clone
- R05-2C3
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 39 kDa; Observed MW: 39 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- ISL LIM Homeobox 1
- Gene ID
- 3670
- UniProt ID
- P61371
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Phosphorylated.
- Protein Refseq
- NP_002193.2
- Function
- DNA-binding transcriptional activator. Recognizes and binds to the consensus octamer binding site 5'-ATAATTAA-3' in promoter of target genes. Plays a fundamental role in the gene regulatory network essential for retinal ganglion cell (RGC) differentiation. Cooperates with the transcription factor POU4F2 to achieve maximal levels of expression of RGC target genes and RGC fate specification in the developing retina. Involved in the specification of motor neurons in cooperation with LHX3 and LDB1. Binds to insulin gene enhancer sequences. Essential for heart development. Marker of one progenitor cell population that give rise to the outflow tract, right ventricle, a subset of left ventricular cells, and a large number of atrial cells as well, its function is required for these progenitors to contribute to the heart. Controls the expression of FGF and BMP growth factors in this cell population and is required for proliferation and survival of cells within pharyngeal foregut endoderm and adjacent splanchnic mesoderm as well as for migration of cardiac progenitors into the heart (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "ISL1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1868 | Hi-Affi™ Recombinant Rabbit Anti-ISL1 Monoclonal Antibody (DS1868AB) | WB, IP, ICC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0875-CN | Recombinant Rabbit Anti-ISL1 Monoclonal Antibody (CBACN-327) | WB, IF, IP | Rabbit IgG |
| ZG-0003U | Rabbit Anti-ISL1 Recombinant Antibody (clone 1A1) | ELISA, WB, IHC | Rabbit IgG |
| VS3-CJ1077 | Rabbit Anti-ISL1 Recombinant Antibody (VS3-CJ1077) | WB, ICC, IF, IP | Rabbit IgG |
| VS7-0425-WR150 | Mouse Anti-ISL1 Monoclonal Antibody (VS7-0425-WR150) | WB, IHC-P, IF, ICC, ELISA | Mouse IgG |
| VS7-0425-WR151 | Mouse Anti-ISL1 Monoclonal Antibody (VS7-0425-WR151) | WB, IF, ICC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC598 | CytoStream™ Rabbit Anti-ISL1 Recombinant Antibody (VS13-YC598) | WB, IHC-P, IHC-Fr, ICC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3604 | Anti-ISL1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3605 | Anti-Mouse ISL1 Immunohistochemistry Kit | IHC |
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