Recombinant Mouse Anti-KRT18 Antibody (VS4-CJ7)
CAT#: VS4-CJ7
This mouse anti-KRT18 antibody is a product that recognizes human, monkey KRT18. The immunogen of this antibody is a purified recombinant human KRT18 protein fragment expressed in E. coli. The mAb has been tested for use in ELISA, Western Blot (1:500-1:5000), Immunocytochemistry (1:50-1:200).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1605/if_selected.jpg
Subcellular Location
Figure 2 IHC staining of human placenta
Immunohistochemical staining of human placenta shows distinct membranous and cytoplasmic positivity in trophoblastic cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1605/ihc_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1605/21_G9_1_blue_red_green.jpg
Subcellular Location
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1605/22_G9_1_blue_red_green.jpg
Normal Tissue
Figure 5 Colon
Endocrine cells
Staining:
High
Intensity: Strong
Enterocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Goblet cells
Staining:
High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30/252_A_8_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Hepatocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30/252_A_9_4.jpg
Normal Tissue
Figure 7 Kidney
Collecting ducts
Staining:
High
Intensity: Strong
Quantity:>75%
Distal tubules
Staining:
High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30/252_A_7_5.jpg
Normal Tissue
Figure 8 Placenta
Cytotrophoblasts
Staining:
High
Intensity: Strong
Quantity:>75%
Endothelial cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Hofbauer cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Syncytiotrophoblasts - cell body
Staining:
High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:
High
Intensity: Strong
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30/252_A_3_7.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (A549, BEWO, CACO-2, CAPAN-2, MCF7, SK-BR-3)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000111057-KRT18
❮
❯
Specifications
- Immunogen
- Purified recombinant human KRT18 protein fragments expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human, Monkey KRT18
- Species Reactivity
- Human, Monkey
- Applications
- ELISA, WB, ICC
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 0.1mg/ml BSA, PBS, pH 7.4.
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at -20°C or -80°C upon receipt. Avoid repeated freeze.
Applications
- Application Notes
- This antibody has been tested for use in ELISA, Western Blot (1:500-1:5000), Immunocytochemistry (1:50-1:200).
Target
- Alternative Names
- K18; CK-18; CYK18
- Gene ID
- 3875
- UniProt ID
- P05783
- Long Name
- Keratin 18
- Sequence Similarities
- Belongs to the intermediate filament family.
- Cellular Localization
- Cytoplasm, Intermediate filament, Keratin, Nucleus
- Post Translation Modifications
- Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation.
- Protein Refseq
- NP_000215.1; NP_954657.1
- Function
- Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS4-CJ7. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "KRT18"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0318MZ | Chicken Anti-KRT18 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1982 | Hi-Affi™ Recombinant Rabbit Anti-KRT18 Monoclonal Antibody (DS1982AB) | IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0408-YC-S(P) | Mouse Anti-KRT18 Recombinant Antibody; scFv Fragment (HPAB-0408-YC-S(P)) | WB | Mouse scFv |
| HPAB-0299-FY-S(P) | Mouse Anti-KRT18 Recombinant Antibody (clone CBLFY07); scFv Fragment | FC, WB, IF, IHC, IP | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0408-YC-F(E) | Mouse Anti-KRT18 Recombinant Antibody; Fab Fragment (HPAB-0408-YC-F(E)) | WB | Mouse Fab |
| HPAB-0299-FY-F(E) | Mouse Anti-KRT18 Recombinant Antibody (clone CBLFY07); Fab Fragment | FC, WB, IF, IHC, IP | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0159 | APC-A*02:01/Human K18 (LIYGTSSRA) MHC Tetramer | FCM | |
| MHC-CN0160 | PE-A*02:01/Human K18 (LIYGTSSRA) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-1079F | Mouse Anti-KRT18 Recombinant Antibody (clone RCK106) | FC, ICC, IHC-Fr, IHC-P, WB | Mouse IgG1 |
| ZG-1085F | Mouse Anti-KRT18 Recombinant Antibody (clone RGE53) | FC, ICC, IHC-Fr, WB | Mouse IgG1 |
| ZG-0323U | Rabbit Anti-KRT18 Recombinant Antibody (clone R20-H) | IHC-P | Rabbit IgG |
| VS3-XY994 | Mouse Anti-KRT18 Recombinant Antibody (clone 2B5-B6-F7) | FC, IF | Mouse IgG2a |
| VS3-XY995 | Mouse Anti-KRT18 Recombinant Antibody (clone 4D11E4) | ELISA, WB, IHC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-CJ422 | Mouse Anti-KRT18 Recombinant Antibody (clone PT1876) | WB, IHC-P | Mouse IgM, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC921 | AbPlus™ Anti-KRT18 Magnetic Beads (VS-0724-YC921) | IP, Protein Purification | |
| VS-0724-YC1035 | AbPlus™ Anti-KRT18 Magnetic Beads (VS-0724-YC1035) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY154 | Mouse Anti-NHP KRT18 Recombinant Antibody (clone C-04 (Ks18.04)) | WB, IHC, IF, FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC634 | CytoStream™ Rabbit Anti-KRT18 Polyclonal Antibody (VS13-YC634) | WB, ELISA, IHC-P, IHC-Fr, FC, ICC, IF | Rabbit IgG |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
Application: Neut, ELISA, IF, IP, FuncS, FC, IHC
-2.png)
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: WB, IF, FuncS
-2.png)
Application: WB, ELISA
Application: Neut, ELISA, FuncS
Application: ELISA, PD, Activ, PK, Stim
Application: ELISA, FC, Inhib
Application: ELISA, Neut, FuncS
Application: ELISA, Inhib, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
