Recombinant Rabbit Anti-KRT19 Antibody (clone R07-1D4)
CAT#: VS3-FY823
This product is a recombinant rabbit antibody that recognizes KRT19. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence. The clone R07-1D4 is specific for human KRT19. The antigen is synthetic peptide of human cytokeratin 19.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2465/1462_C1_1_selected.jpg
Normal Tissue
Figure 2 Urinary bladder
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2465/8762_A_5_5_val_selected.jpg
Normal Tissue
Figure 3 Colon
Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity: 75%-25% Enterocytes - Gradient Gradient: Ascending Enterocytes - Microvilli Staining: High Intensity: Strong Goblet cells Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2465/8762_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2465/8762_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Bowman's capsule Staining: Medium Intensity: Strong Quantity: <25% Collecting ducts Staining: High Intensity: Strong Quantity:>75% Distal tubules Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2465/8762_A_8_5.jpg
Normal Tissue
Figure 6 Breast
Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2465/8762_B_3_4.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (CAPAN-2, HBEC3-KT, MCF7, RT4, SK-BR-3, T-47d)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000171345-KRT19
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WB
Figure 1. Recombinant Rabbit Anti-KRT19 Antibody (clone R07-1D4) in WB.
Western Blot analysis of cytokeratin 19 in K562 lysates using a cytokeratin 19 antibody.
IHC-P
Figure 2. Recombinant Rabbit Anti-KRT19 Antibody (clone R07-1D4) in IHC-P.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using an antibody to cytokeratin 19. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Specifications
- Immunogen
- Synthetic peptide of human cytokeratin 19.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human KRT19
- Species Reactivity
- Human
- Clone
- R07-1D4
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- Calculated MW: 44 kDa; Observed MW: 41 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "KRT19"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1983 | Hi-Affi™ Recombinant Rabbit Anti-KRT19 Monoclonal Antibody (DS1983AB) | FC, ICC, IHC-P, IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-024CN | Mouse Anti-KRT19 Recombinant Antibody (clone 11F5) | ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-024CN-F(E) | Mouse Anti-KRT19 Recombinant Antibody (clone 11F5); Fab Fragment | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-024CN-S(P) | Mouse Anti-KRT19 Recombinant Antibody (clone 11F5); scFv Fragment | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0463F | Mouse Anti-KRT19 Recombinant Antibody (ZG-0463F) | WB, ELISA | Mouse IgG |
| ZG-0246J | Mouse Anti-KRT19 Recombinant Antibody (ZG-0246J) | IHC-P, IF | Mouse IgG |
| ZG-0247J | Mouse Anti-KRT19 Recombinant Antibody (ZG-0247J) | IHC-P, IF | Mouse IgG |
| ZG-0177U | Mouse Anti-KRT19 Recombinant Antibody (clone 2B1D6) | ELISA, WB, IHC | Mouse IgG2a |
| ZG-0206U | Mouse Anti-KRT19 Recombinant Antibody (clone 1C1B3) | ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1429 | AbPlus™ Anti-KRT19 Magnetic Beads (VS-0724-YC1429) | IP, Protein Purification | |
| VS-0724-YC1430 | AbPlus™ Anti-KRT19 Magnetic Beads (VS-0724-YC1430) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1214 | Anti-KRT19 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3862 | Anti-Mouse KRT19 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3864 | Anti-Rat KRT19 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3861 | Anti-Human KRT19 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC635 | CytoStream™ Rabbit Anti-KRT19 Polyclonal Antibody (VS13-YC635) | WB, ELISA, IHC-P, IHC-Fr, FC, ICC, IF | Rabbit IgG |
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