Recombinant Rabbit Anti-MAP2K1 Antibody (clone R01-3C2)
CAT#: VS3-FY877
This product is a recombinant rabbit antibody that recognizes MAP2K1. This antibody has been reported for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The clone R01-3C2 is specific for human MAP2K1. The antigen is synthetic peptide of human mek1.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26430/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3834/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human hippocampus
Immunohistochemical staining of human hippocampus shows moderate cytoplasmic positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26430/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human stomach
Immunohistochemical staining of human stomach shows moderate cytoplasmic and nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3834/ihc_selected.jpg
Normal Tissue
Figure 5 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26430/57722_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26430/57722_A_8_3.jpg
Normal Tissue
Figure 7 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26430/57722_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000169032-MAP2K1
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ICC
Figure 1. Recombinant Rabbit Anti-MAP2K1 Antibody (clone R01-3C2) in ICC.
Immunocytochemical analysis of MEK1 (green) in HeLa using a MEK1 antibody and DAPI (blue).
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Specifications
- Immunogen
- Synthetic peptide of human MEK1.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human MAP2K1
- Species Reactivity
- Human
- Clone
- R01-3C2
- Applications
- Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 43 kDa; Observed MW: 43 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "MAP2K1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1129CT | Recombinant Mouse anti-Human MAP2K1 Monoclonal antibody (8G2) | IP, WB | |
| MRO-0978-CN | Recombinant Rabbit Anti-MAP2K1 Monoclonal Antibody (CBACN-357) | WB, IF, IHC, IP, FC | Rabbit IgG |
| ZG-0451U | Rabbit Anti-Phospho-MAP2K1 (T292) Recombinant Antibody (clone 4E7) | ELISA, WB | Rabbit IgG |
| ZG-0452U | Rabbit Anti-MAP2K1 Recombinant Antibody (clone 3F10) | ELISA, WB, IHC, IP | Rabbit IgG |
| VS3-FY2793 | Rabbit Anti-MAP2K1 Recombinant Antibody (VS3-FY2793) | WB, IHC, ICC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0340MZ | Chicken Anti-MAP2K1 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2131 | Hi-Affi™ Recombinant Rabbit Anti-MAP2K1 Monoclonal Antibody (DS2131AB) | ICC, IHC-P, IP, WB | IgG |
| MOR-4693 | Hi-Affi™ Recombinant Rabbit Anti-MAP2K1 Monoclonal Antibody (TH207DS) | WB, ChIP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY180 | AbPlus™ Anti-MAP2K1 Magnetic Beads (CBACN-357) | IP, Protein Purification | |
| VS-0724-YC941 | AbPlus™ Anti-MAP2K1 Magnetic Beads (VS-0724-YC941) | IP, Protein Purification | |
| VS-0724-YC968 | AbPlus™ Anti-Map2k1 Magnetic Beads (VS-0724-YC968) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4205 | Anti-MAP2K1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4206 | Anti-Mouse MAP2K1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4207 | Anti-Rat MAP2K1 Immunohistochemistry Kit | IHC |
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