Recombinant Mouse Anti-MCM3 Antibody (clone 1D4A3) (CAT#: VS3-FY911)
This product is a recombinant mouse antibody that recognizes MCM3. This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry. The clone 1D4A3 is specific for MCM3.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & centrosome.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4790/13_D12_2_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & centrosome.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4790/13_D12_2_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2162/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2162/if_selected.jpg
Figure 3 IHC staining of human tonsil
(Immunohistochemical staining of human tonsil shows strong nuclear positivity in reaction center cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4789/ihc_selected.jpg
(Immunohistochemical staining of human tonsil shows strong nuclear positivity in reaction center cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4789/ihc_selected.jpg
Figure 4 IHC staining of human lymph node
(Immunohistochemical staining of human lymph node shows strong nuclear positivity in reaction center cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4790/ihc_selected.jpg
(Immunohistochemical staining of human lymph node shows strong nuclear positivity in reaction center cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4790/ihc_selected.jpg
Figure 5 Colon
(Glandular cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2162/5734_A_8_3.jpg
(Glandular cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2162/5734_A_8_3.jpg
Figure 6 Testis
(Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2162/5734_A_6_6.jpg
(Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2162/5734_A_6_6.jpg
Figure 7 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4789/15794_A_8_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4789/15794_A_8_8.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000112118-MCM3
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000112118-MCM3
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG1, κ
- Clone
- 1D4A3
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry
- Conjugate
- Unconjugated
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry.
Target
- Alternative Names
- Minichromosome Maintenance Complex Component 3; DNA Polymerase Alpha Holoenzyme-Associated Protein P1; RLF Subunit Beta; P1-MCM3; P102; MCM3 Minichromosome Maintenance Deficient 3 (S. Cerevisiae); Minichromosome Maintenance Deficient (S. Cerevisiae) 3; MCM3 Minichromosome Maintenance Deficient 3; Replication Licensing Factor, Beta Subunit; Minichromosome Maintenance Deficient 3
- Gene ID
- 4172
- UniProt ID
- P25205
- Sequence Similarities
- Belongs to the MCM family.
- Cellular Localization
- Chromosome, Nucleus
- Post Translation Modifications
- Acetylated by MCM3AP.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
- Protein Refseq
- NP_001257401.1; NP_002379.3
- Function
- Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed:32453425, PubMed:34694004, PubMed:34700328, PubMed:35585232).
The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed:32453425).
Required for the entry in S phase and for cell division (Probable).
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2628z | Mouse Anti-MCM3 Recombinant Antibody (clone 35B1) | WB, ICC, IF, IHC, IP | Mouse IgG1, κ |
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| VS3-WK339 | Rabbit Anti-MCM3 Recombinant Antibody (clone JB99-35) | WB, ELISA | Rabbit IgG |
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