Recombinant Mouse Anti-MKI67 Antibody (VS4-CJ36) (CAT#: VS4-CJ36)
This mouse anti-MKI67 antibody is a product that recognizes human, mouse, rat MKI67. The immunogen of this antibody is a synthetic peptide corresponding to MKI67. The mAb has been tested for use in ELISA, Immunohistochemistry (1:100-1:200).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear bodies & nucleoli rim.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1164/13_B8_2_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear bodies & nucleoli rim.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1164/13_B8_2_selected.jpg
Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nucleoli rim.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58/952_H5_3_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nucleoli rim.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58/952_H5_3_selected.jpg
Figure 3 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nucleoli rim.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1164/30_B1_1_red_green.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nucleoli rim.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1164/30_B1_1_red_green.jpg
Figure 4 Colon
(Goblet cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58/155454_A_7_3.jpg
(Goblet cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58/155454_A_7_3.jpg
Figure 5 Testis
(Cells in seminiferous ducts
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58/155454_A_6_6.jpg
(Cells in seminiferous ducts
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58/155454_A_6_6.jpg
Figure 6 Skin
(Cells in basal layer
Staining: Medium
Intensity: Strong
Quantity: <25%
Cells in spinous layer
Staining: Medium
Intensity: Strong
Quantity: <25%
Hair follicles
Staining: High
Intensity: Strong
Quantity: 75%-25%
Lymphocytes
Staining: High
Intensity: Strong
Sebaceous glands
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58/155454_B_7_1.jpg
(Cells in basal layer
Staining: Medium
Intensity: Strong
Quantity: <25%
Cells in spinous layer
Staining: Medium
Intensity: Strong
Quantity: <25%
Hair follicles
Staining: High
Intensity: Strong
Quantity: 75%-25%
Lymphocytes
Staining: High
Intensity: Strong
Sebaceous glands
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58/155454_B_7_1.jpg
Figure 7 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58/155454_A_8_8.jpg
(Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58/155454_A_8_8.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000148773-MKI67
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000148773-MKI67
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000148773-MKI67
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000148773-MKI67
Specifications
- Immunogen
- Synthetic Peptide
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human, Mouse, Rat MKI67
- Species Reactivity
- Human, Mouse, Rat
- Applications
- ELISA, IHC
- Conjugate
- Unconjugated
Product Property
- Purification
- Immunogen affinity chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, PBS, pH 7.4.
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at -20°C or -80°C upon receipt. Avoid repeated freeze.
Applications
- Application Notes
- This antibody has been tested for use in ELISA, Immunohistochemistry (1:100-1:200).
Target
- Alternative Names
- KIA; MIB-; MIB-1; PPP1R105
- Gene ID
- 4288
- UniProt ID
- P46013
- Long Name
- Marker of proliferation Ki-67
- Cellular Localization
- Chromosome, Nucleus
- Post Translation Modifications
- Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604).
Hyperphosphorylated form does not bind DNA.
- Protein Refseq
- NP_001139438.1; NP_002408.3
- Function
- Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226).
Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226).
Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226).
Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551).
Does not contribute to the internal structure of mitotic chromosomes (By similarity).
May play a role in chromatin organization (PubMed:24867636).
It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1072z | Mouse Anti-MKI67 Recombinant Antibody (clone 26F12) | WB, ELISA, IHC | Mouse IgG1 |
| MRO-1993-CN | Rabbit Anti-MKI67 Polyclonal Antibody (MRO-1993-CN) | WB, IF, IHC, FC | Rabbit IgG |
| MRO-1994-CN | Rabbit Anti-MKI67 Polyclonal Antibody (MRO-1994-CN) | WB, IHC | Rabbit IgG |
| ZG-0240C | Mouse Anti-MKI67 Recombinant Antibody (ZG-0240C) | IHC, ELISA | Mouse IgG |
| ZG-0241C | Mouse Anti-MKI67 Recombinant Antibody (ZG-0241C) | WB, ELISA | Mouse IgG |
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