Recombinant Rabbit Anti-MYL2 Antibody (clone R07-7C5) (CAT#: VS3-FY994)

• Immunogen
• Synthetic peptide of human myosin light chain 2.

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Mouse, Rat MYL2

• Species Reactivity
• Mouse, Rat

• Clone
• R07-7C5

• Applications
• Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation

• Conjugate
• Unconjugated

• MW
• Calculated MW: 19 kDa; Observed MW: 19 kDa

• Purification
• Affinity Purified

• Purity
• >95% as determined by SDS-PAGE

• Buffer
• 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA

• Preservative
• 0.01% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunohistochemistry-Paraffin: 1/50-1/100
  Immunoprecipitation: 1/20

• Alternative Names
• Myosin Light Chain 2; Myosin Light Chain 2, Slow Skeletal/Ventricular Muscle Isoform; Myosin, Light Polypeptide 2, Regulatory, Cardiac, Slow; Myosin, Light Chain 2, Regulatory, Cardiac, Slow; Cardiac Ventricular Myosin Light Chain 2; Ventricular Myosin Light Chain 2; Cardiac Myosin Light Chain 2; MLC-2s/V; MLC-2v

• Gene ID
• 4633

• UniProt ID
• P10916

• Cellular Localization
• Cytoplasm

• Post Translation Modifications
• N-terminus is methylated by METTL11A/NTM1.
  Phosphorylated by MYLK3 and MYLK2; promotes cardiac muscle contraction and function. Dephosphorylated by PPP1CB complexed to PPP1R12B (By similarity).
  The phosphorylated form in adult is expressed as gradients across the heart from endocardium (low phosphorylation) to epicardium (high phosphorylation); regulates cardiac torsion and workload distribution (PubMed:22426213).

• Protein Refseq
• NP_034991.3

• Function
• Contractile protein that plays a role in heart development and function (PubMed:10409661).
  Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion; as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events altogether slow down myosin kinetics and prolong duty cycle resulting in accumulated myosins being cooperatively recruited to actin binding sites to sustain thin filament activation as a means to fine-tune myofilament calcium sensitivity to force (By similarity) (PubMed:22426213, PubMed:16908724, PubMed:10409661).
  During cardiogenesis plays an early role in cardiac contractility by promoting cardiac myofibril assembly (PubMed:9422794).

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody

• 2ND-057CQ
• Donkey Anti-Rabbit IgG (H&L) Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-060CQ
• Llama Anti-Rabbit IgG (H&L) Antibody

• 2ND-061CQ
• Sheep Anti-Rabbit IgG (H&L) Antibody

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody