Recombinant Mouse Anti-NEFL Antibody (clone 2D7-6C10-10B2)
CAT#: VS3-FY1026
This product is a recombinant mouse antibody that recognizes NEFL. This antibody has been reported for use in Immunohistochemistry-Paraffin. The clone 2D7-6C10-10B2 is specific for human NEFL. The antigen is synthetic peptides derived from human neurofilament.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm, intermediate filaments & midbody.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15021/1910_D3_1_selected.jpg
Normal Tissue
Figure 2 Low expression in tonsil
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14850/165171_A_6_8_val_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuropil Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14850/165171_B_8_5.jpg
RNA Expression
Figure 4 RNA cell line category: Group enriched (HEK93, PC-3)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000277586-NEFL
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IHC-P
Figure 1. Recombinant Mouse Anti-NEFL Antibody (clone 2D7-6C10-10B2) in IHC-P.
Immunohistochemical analysis of paraffin-embedded brain using Neurofilament Light Polypeptide Antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Specifications
- Immunogen
- Synthetic peptides derived from human neurofilament.
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human NEFL
- Species Reactivity
- Human
- Clone
- 2D7-6C10-10B2
- Applications
- Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity Chromatography
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS, pH 7.3, 50% glycerol, 0.5% BSA
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry-Paraffin: 1/100-1/500
Target
- Alternative Names
- Neurofilament Light; Protein Phosphatase 1, Regulatory Subunit 110; Neurofilament, Light Polypeptide 68kDa; Neurofilament Triplet L Protein; NF-L; NF68; NFL; Light Molecular Weight Neurofilament Protein; Neurofilament Protein, Light Chain
- Gene ID
- 4747
- UniProt ID
- P07196
- Sequence Similarities
- Belongs to the intermediate filament family.
- Cellular Localization
- Cell projection, Cytoplasm, Cytoskeleton, Intermediate filament
- Post Translation Modifications
- O-glycosylated.
Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.
Ubiquitinated in the presence of TRIM2 and UBE2D1.
- Protein Refseq
- NP_006149.2
- Function
- Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
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Reconstitution Buffer
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1158CT | Recombinant Mouse anti-Rat NEFL Monoclonal antibody (2E3) | ICC, IF, IHC-Fr, IHC-P, WB | |
| ZG-0563F | Mouse Anti-NEFL Recombinant Antibody (clone 2F11) | IHC-Fr, IHC-P, IB | Mouse IgG1 |
| VS3-XY1150 | Mouse Anti-NEFL Recombinant Antibody (clone 2G10) | ELISA, WB, IHC, FC, ICC | Mouse IgG1 |
| VS3-FY1025 | Recombinant Rabbit Anti-Nefl Antibody (clone R04-7H3) | WB, IHC-P, IP | Rabbit IgG |
| VS7-0425-WR462 | Mouse Anti-NEFL Recombinant Antibody (clone 2F8B3) | WB, IHC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0822MZ | Chicken Anti-NEFL Polyclonal IgY | ICC, IF, IHC, WB | Chicken antibody |
| BRD-1153MZ | Chicken Anti-Neurofilament Light Polyclonal IgY | WB, ICC | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2411 | Hi-Affi™ Recombinant Rabbit Anti-NEFL Monoclonal Antibody (DS2411AB) | WB, IP, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC782 | CytoStream™ Mouse Anti-NEFL Recombinant Antibody (VS13-YC782) | WB, IHC-P, FC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4777 | Anti-NEFL Immunohistochemistry Kit | IHC | |
| VS-0525-XY4778 | Anti-Mouse NEFL Immunohistochemistry Kit | IHC | |
| VS-0525-XY4780 | Anti-Rat NEFL Immunohistochemistry Kit | IHC |
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