Recombinant Rabbit Anti-NEK6 Antibody (clone R01-4F7) (CAT#: VS3-FY1027)
This product is a recombinant rabbit antibody that recognizes NEK6. This antibody has been reported for use in Western Blot. The clone R01-4F7 is specific for human, rat NEK6. The antigen is synthetic peptide of human nek6.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HeLa
(Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56828/1050_E7_1_selected.jpg
(Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56828/1050_E7_1_selected.jpg
Figure 2 IHC staining of human stomach, lower
(Immunohistochemical staining of human stomach, lower shows strong granular cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/45993/ihc_selected.jpg
(Immunohistochemical staining of human stomach, lower shows strong granular cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/45993/ihc_selected.jpg
Figure 3 Cerebral cortex
(Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/45993/111150_B_9_5.jpg
(Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/45993/111150_B_9_5.jpg
Figure 4 Gallbladder
(Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/45993/111150_A_6_4.jpg
(Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/45993/111150_A_6_4.jpg
Figure 5 Kidney
(Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/45993/111150_A_9_5.jpg
(Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/45993/111150_A_9_5.jpg
Figure 6 Testis
(Round or early spermatids Staining: Medium Intensity: Strong Quantity: <25% Spermatogonia cells Staining: Low Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/45993/111150_A_4_6.jpg
(Round or early spermatids Staining: Medium Intensity: Strong Quantity: <25% Spermatogonia cells Staining: Low Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/45993/111150_A_4_6.jpg
Figure 7 RNA cell line category: Cell line enhanced (HMC-1)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000119408-NEK6
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000119408-NEK6
Specifications
- Immunogen
- Synthetic peptide of human NEK6.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Rat NEK6
- Species Reactivity
- Human, Rat
- Clone
- R01-4F7
- Applications
- Western Blot
- Conjugate
- Unconjugated
- MW
- Calculated MW: 36 kDa; Observed MW: 36 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- NIMA Related Kinase 6; NIMA (Never In Mitosis Gene A)-Related Kinase 6; Putative Serine-Threonine Protein Kinase; Never In Mitosis A-Related Kinase 6; NimA-Related Protein Kinase 6; Protein Kinase SID6-1512; EC 2.7.11.1; Serine/Threonine-Protein Kinase Nek6; SID6-1512
- Gene ID
- 10783
- UniProt ID
- Q9HC98
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibody Products
IgG Antibody Products
IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2417 | Hi-Affi™ Recombinant Rabbit Anti-NEK6 Monoclonal Antibody (DS2417AB) | WB | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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