Recombinant Rabbit Anti-NEK6 Antibody (clone R08-5G7) (CAT#: VS3-FY1028)
This product is a recombinant rabbit antibody that recognizes NEK6. This antibody has been reported for use in Western Blot, Immunoprecipitation. The clone R08-5G7 is specific for human NEK6. The antigen is synthetic peptide of human nek6.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HeLa
(Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56828/1050_E7_1_selected.jpg
(Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56828/1050_E7_1_selected.jpg
Figure 2 IHC staining of human stomach, lower
(Immunohistochemical staining of human stomach, lower shows strong granular cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/45993/ihc_selected.jpg
(Immunohistochemical staining of human stomach, lower shows strong granular cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/45993/ihc_selected.jpg
Figure 3 Cerebral cortex
(Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/45993/111150_B_9_5.jpg
(Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/45993/111150_B_9_5.jpg
Figure 4 Gallbladder
(Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/45993/111150_A_6_4.jpg
(Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/45993/111150_A_6_4.jpg
Figure 5 Kidney
(Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/45993/111150_A_9_5.jpg
(Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/45993/111150_A_9_5.jpg
Figure 6 Testis
(Round or early spermatids Staining: Medium Intensity: Strong Quantity: <25% Spermatogonia cells Staining: Low Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/45993/111150_A_4_6.jpg
(Round or early spermatids Staining: Medium Intensity: Strong Quantity: <25% Spermatogonia cells Staining: Low Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/45993/111150_A_4_6.jpg
Figure 7 RNA cell line category: Cell line enhanced (HMC-1)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000119408-NEK6
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000119408-NEK6
Specifications
- Immunogen
- Synthetic peptide of human NEK6.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human NEK6
- Species Reactivity
- Human
- Clone
- R08-5G7
- Applications
- Western Blot, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 36 kDa; Observed MW: 36 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- NIMA Related Kinase 6; NIMA (Never In Mitosis Gene A)-Related Kinase 6; Putative Serine-Threonine Protein Kinase; Never In Mitosis A-Related Kinase 6; NimA-Related Protein Kinase 6; Protein Kinase SID6-1512; EC 2.7.11.1; Serine/Threonine-Protein Kinase Nek6; SID6-1512
- Gene ID
- 10783
- UniProt ID
- Q9HC98
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibodies
IgG Antibodies
IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2417 | Hi-Affi™ Recombinant Rabbit Anti-NEK6 Monoclonal Antibody (DS2417AB) | WB | IgG |
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For Research Use Only. Not For Clinical Use.
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