Recombinant Rabbit Anti-NFYA Antibody (clone R01-5G1)
CAT#: VS3-FY1040
This product is a recombinant rabbit antibody that recognizes NFYA. This antibody has been reported for use in Western Blot, Immunoprecipitation. The clone R01-5G1 is specific for human, mouse, rat NFYA. The antigen is human nfya synthetic peptide.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50779/835_D10_1_selected.jpg
Normal Tissue
Figure 2 IHC staining of human stomach, lower
Immunohistochemical staining of human stomach, Lower shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50779/ihc_selected.jpg
Normal Tissue
Figure 3 Bone marrow.
High expression in bone marrow
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9250/24621_B_6_4_val_selected.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9250/24621_A_7_8.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50779/123398_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50779/123398_A_7_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50779/123398_A_7_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50779/123398_A_8_5.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000001167-NFYA
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WB
Figure 1. Recombinant Rabbit Anti-NFYA Antibody (clone R01-5G1) in WB.
Western Blot analysis of NFYA in C6, 3T3, Hela lysates using NFYA antibody.
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Specifications
- Immunogen
- Human NFYA Synthetic Peptide.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat NFYA
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R01-5G1
- Applications
- Western Blot, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 37 kDa; Observed MW: 37 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- Nuclear Transcription Factor Y Subunit Alpha; Nuclear Transcription Factor Y Subunit A; Nuclear Transcription Factor Y, Alpha; NF-YA; CCAAT-Binding Transcription Factor Subunit B; CAAT-Box DNA Binding Protein Subunit A; CAAT Box DNA-Binding Protein Subunit A
- Gene ID
- 4800
- UniProt ID
- P23511
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "NFYA"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3146z | Mouse Anti-NFYA Recombinant Antibody (clone 42B8) | WB, IP, IF, IHC, ELISA | Mouse IgG2a, κ |
| VS13-YC804 | Rabbit Anti-NFYA Recombinant Antibody (VS13-YC804) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2438 | Hi-Affi™ Recombinant Rabbit Anti-NFYA Monoclonal Antibody (DS2438AB) | WB, ICC, IP, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC744 | AbPlus™ Anti-NFYA Magnetic Beads (VS-0724-YC744) | IP, Protein Purification |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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