Recombinant Mouse Anti-NPM1 Antibody (VS4-CJ58)
CAT#: VS4-CJ58
This mouse anti-NPM1 antibody is a product that recognizes human, rabbit, porcine, rat, mouse NPM1. The immunogen of this antibody is full length recombinant protein (295aa) of human NPM1. The mAb has been tested for use in ELISA, Western Blot (1:500-1:5000), Immunocytochemistry (1:50-1:200).
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nucleoli rim.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11384/108_B11_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human colon
Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11384/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows strong nuclear positivity in cerebellar tissue.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12983/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12983/28235_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12983/28235_A_9_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11384/28259_A_8_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12983/28235_A_8_5.jpg
Normal Tissue
Figure 8 Testis
Pachytene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Peritubular cells
Staining: High
Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Round or early spermatids
Staining: High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining: High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12983/28235_A_6_6.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12983/28235_A_7_8.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000181163-NPM1
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Specifications
- Immunogen
- Full length recombinant protein (295aa) of human NPM1.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human, Rabbit, Porcine, Rat, Mouse NPM1
- Species Reactivity
- Human, Rabbit, Porcine, Rat, Mouse
- Applications
- ELISA, WB, ICC
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 0.1M Tris-Glycine (150 mM NaCl), pH 7.4.
- Preservative
- 0.2% Sodium Azide
- Storage
- Store at -20°C or -80°C upon receipt. Avoid repeated freeze.
Applications
- Application Notes
- This antibody has been tested for use in ELISA, Western Blot (1:500-1:5000), Immunocytochemistry (1:50-1:200).
Target
- Alternative Names
- B23; NPM
- Gene ID
- 4869
- UniProt ID
- P06748
- Long Name
- Nucleophosmin 1
- Sequence Similarities
- Belongs to the nucleoplasmin family.
- Cellular Localization
- Cytoplasm, Cytoskeleton, Nucleus
- Post Translation Modifications
- Acetylated at C-terminal lysine residues, thereby increasing affinity to histones.
ADP-ribosylated.
Phosphorylated at Ser-4 by PLK1 and PLK2. Phosphorylation at Ser-4 by PLK2 in S phase is required for centriole duplication and is sufficient to trigger centriole replication. Phosphorylation at Ser-4 by PLK1 takes place during mitosis. Phosphorylated by CDK2 at Ser-125 and Thr-199. Phosphorylation at Thr-199 may trigger initiation of centrosome duplication. Phosphorylated by CDK1 at Thr-199, Thr-219, Thr-234 and Thr-237 during cell mitosis. When these four sites are phosphorated, RNA-binding activity seem to be abolished. May be phosphorylated at Ser-70 by NEK2. The Thr-199 phosphorylated form has higher affinity for ROCK2. CDK6 triggers Thr-199 phosphorylation when complexed to Kaposi's sarcoma herpesvirus (KSHV) V-cyclin, leading to viral reactivation by reducing viral LANA levels.
Sumoylated by ARF.
Ubiquitinated. Ubiquitination leads to proteasomal degradation. Deubiquitinated by USP36 (PubMed:19208757).
- Protein Refseq
- NP_001032827.1; NP_002511.1; NP_954654.1
- Function
- Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double- stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation.Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade (PubMed:22528486).
In complex with MYC enhances the transcription of MYC target genes (PubMed:25956029).
May act as chaperonin or cotransporter in the nucleolar localization of transcription termination factor TTF1 (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "NPM1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-711 | Recombinant Anti-human NPM1 Antibody | ELISA, WB, IP, FuncS | IgG |
| MHH-711 | Recombinant Human Anti-human NPM1 Antibody | ELISA, WB, FC, FuncS | IgG |
| ZG-0319C | Mouse Anti-NPM1 Recombinant Antibody (ZG-0319C) | WB, ICC | Mouse IgG |
| VS3-QX827 | Mouse Anti-NPM1 Recombinant Antibody (clone 2D9-C3-E5) | WB | Mouse IgG1 |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-711-F(E) | Recombinant Anti-human NPM1 Antibody Fab Fragment | ELISA, FuncS | Fab |
| MHH-711-F(E) | Recombinant Human Anti-human NPM1 Antibody Fab Fragment | FC, IP, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-711-S(P) | Recombinant Anti-human NPM1 Antibody scFv Fragment | WB, IHC, FuncS | scFv |
| MHH-711-S(P) | Recombinant Human Anti-human NPM1 Antibody scFv Fragment | FC, Neut, Biosensors, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2480 | Hi-Affi™ Recombinant Rabbit Anti-NPM1 Monoclonal Antibody (DS2480AB) | ICC, IF, IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-YF593 | A*02:01/Human NPM1 (AIQDLCLAV) MHC Pentamer | FCM | |
| MHC-YF594 | A*02:01/Human NPM1 (AIQDLCVAV) MHC Pentamer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC431 | AbPlus™ Anti-NPM1 Magnetic Beads (VS-0724-YC431) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC82 | Rabbit Anti-NPM1 Antibody (VS-0924-YC82) - Cancer Stem Cell Marker | IHC, WB, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY346 | Mouse Anti-NHP NPM1 Recombinant Antibody (clone 7H10B9) | WB, IHC, IF, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1511 | Anti-NPM1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4922 | Anti-Mouse NPM1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4921 | Anti-Human NPM1 Immunohistochemistry Kit | IHC |
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