Recombinant Rabbit Anti-OCLN Antibody (clone 8H8)
CAT#: VS4-CJ64
This rabbit anti-OCLN antibody (clone 8H8) is a product that recognizes human OCLN. The immunogen of this antibody is a synthesized peptide derived from human OCLN. The mAb has been tested for use in ELISA, Immunohistochemistry (1:50-1:200), Flow Cytometry (1:50-1:200).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cell junctions.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5933/if_selected.jpg
Normal Tissue
Figure 2 Thyroid gland
High expression in thyroid gland
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5933/15984_B_1_5_val_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13075/32513_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13075/32314_A_9_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13075/32314_A_7_4.jpg
Normal Tissue
Figure 6 Gallbladder
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13075/32314_A_6_4.jpg
Normal Tissue
Figure 7 Kidney
Collecting ducts Staining: Medium Intensity: Moderate Quantity:>75% Distal tubules Staining: Medium Intensity: Moderate Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13075/32314_A_8_5.jpg
Normal Tissue
Figure 8 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13075/32314_A_6_6.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (A-431, CAPAN-2, HaCaT, RPTEC TERT1, SK-BR-3)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000197822-OCLN
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Specifications
- Immunogen
- A synthesized peptide derived from human OCLN
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human OCLN
- Species Reactivity
- Human
- Clone
- 8H8
- Applications
- ELISA, IHC, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 150 mM NaCl, PBS, pH 7.4.
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at -20°C or -80°C upon receipt. Avoid repeated freeze.
Applications
- Application Notes
- This antibody has been tested for use in ELISA, Immunohistochemistry (1:50-1:200), Flow Cytometry (1:50-1:200).
Target
- Alternative Names
- BLCPMG; PTORCH1; PPP1R115
- Gene ID
- 100506658
- UniProt ID
- Q16625
- Long Name
- Occludin
- Sequence Similarities
- Belongs to the ELL/occludin family.
- Cellular Localization
- Cell junction, Cell membrane, Membrane, Tight junction
- Post Translation Modifications
- Dephosphorylated by PTPRJ. The tyrosine phosphorylation on Tyr-398 and Tyr-402 reduces its ability to interact with TJP1. Phosphorylation at Ser-490 also attenuates the interaction with TJP1.
(Microbial infection) Cleaved by S.pyogenes SpeB protease; leading to its degradation (PubMed:23532847).
Degradation by SpeB promotes bacterial translocation across the host epithelial barrier (PubMed:23532847).
- Protein Refseq
- NP_001192183.1; NP_001192184.1; NP_002529.1
- Function
- May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.
(Microbial infection) Acts as a coreceptor for hepatitis C virus (HCV) in hepatocytes.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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