Recombinant Rabbit Anti-OCLN Antibody (clone 8H8) (CAT#: VS4-CJ64)
This rabbit anti-OCLN antibody (clone 8H8) is a product that recognizes human OCLN. The immunogen of this antibody is a synthesized peptide derived from human OCLN. The mAb has been tested for use in ELISA, Immunohistochemistry (1:50-1:200), Flow Cytometry (1:50-1:200).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cell junctions.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5933/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cell junctions.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5933/if_selected.jpg
Figure 2 Thyroid gland
(High expression in thyroid gland)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5933/15984_B_1_5_val_selected.jpg
(High expression in thyroid gland)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5933/15984_B_1_5_val_selected.jpg
Figure 3 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13075/32513_B_7_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13075/32513_B_7_5.jpg
Figure 4 Colon
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13075/32314_A_9_3.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13075/32314_A_9_3.jpg
Figure 5 Liver
(Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13075/32314_A_7_4.jpg
(Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13075/32314_A_7_4.jpg
Figure 6 Gallbladder
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13075/32314_A_6_4.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13075/32314_A_6_4.jpg
Figure 7 Kidney
(Collecting ducts Staining: Medium Intensity: Moderate Quantity:>75% Distal tubules Staining: Medium Intensity: Moderate Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13075/32314_A_8_5.jpg
(Collecting ducts Staining: Medium Intensity: Moderate Quantity:>75% Distal tubules Staining: Medium Intensity: Moderate Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13075/32314_A_8_5.jpg
Figure 8 Testis
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13075/32314_A_6_6.jpg
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13075/32314_A_6_6.jpg
Figure 9 RNA cell line category: Cell line enhanced (A-431, CAPAN-2, HaCaT, RPTEC TERT1, SK-BR-3)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000197822-OCLN
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000197822-OCLN
Specifications
- Immunogen
- A synthesized peptide derived from human OCLN
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human OCLN
- Species Reactivity
- Human
- Clone
- 8H8
- Applications
- ELISA, IHC, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 150 mM NaCl, PBS, pH 7.4.
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at -20°C or -80°C upon receipt. Avoid repeated freeze.
Applications
- Application Notes
- This antibody has been tested for use in ELISA, Immunohistochemistry (1:50-1:200), Flow Cytometry (1:50-1:200).
Target
- Alternative Names
- BLCPMG; PTORCH1; PPP1R115
- Gene ID
- 100506658
- UniProt ID
- Q16625
- Long Name
- Occludin
- Sequence Similarities
- Belongs to the ELL/occludin family.
- Cellular Localization
- Cell junction, Cell membrane, Membrane, Tight junction
- Post Translation Modifications
- Dephosphorylated by PTPRJ. The tyrosine phosphorylation on Tyr-398 and Tyr-402 reduces its ability to interact with TJP1. Phosphorylation at Ser-490 also attenuates the interaction with TJP1.
(Microbial infection) Cleaved by S.pyogenes SpeB protease; leading to its degradation (PubMed:23532847).
Degradation by SpeB promotes bacterial translocation across the host epithelial barrier (PubMed:23532847).
- Protein Refseq
- NP_001192183.1; NP_001192184.1; NP_002529.1
- Function
- May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.
(Microbial infection) Acts as a coreceptor for hepatitis C virus (HCV) in hepatocytes.
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2734z | Mouse Anti-OCLN Recombinant Antibody (clone 34D5) | WB, ELISA, IF, IHC | Mouse IgG1, κ |
| MRO-1124-CN | Recombinant Rabbit Anti-OCLN Monoclonal Antibody (CBACN-410) | WB, IP, FC | Rabbit IgG |
| MRO-2091-CN | Rabbit Anti-OCLN Polyclonal Antibody (MRO-2091-CN) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-WK1012 | Rabbit Anti-OCLN Recombinant Antibody (VS3-WK1012) | WB, IP, FC | Rabbit IgG |
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