Recombinant Rabbit Anti-PFKFB3 Antibody (clone R08-7A4)
CAT#: VS3-FY1124
This product is a recombinant rabbit antibody that recognizes PFKFB3. This antibody has been reported for use in Western Blot, Immunoprecipitation. The clone R08-7A4 is specific for human, mouse, rat PFKFB3. The antigen is human pfkfb3 recombinant protein.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8266/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/43889/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human rectum
Immunohistochemical staining of human rectum shows moderate cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20795/48008_A_5_3_selected.jpg
Normal Tissue
Figure 4 Lymph node
Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20795/48008_A_7_8.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20795/48008_A_7_5.jpg
Normal Tissue
Figure 6 Bone marrow
Hematopoietic cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20795/48008_B_6_4.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (A549, RPTEC TERT1, T-47d)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000170525-PFKFB3
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WB
Figure 1. Recombinant Rabbit Anti-PFKFB3 Antibody (clone R08-7A4) in WB.
Western Blot analysis of PFKFB3 in K562 lysates using PFKFB3 antibody.
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Specifications
- Immunogen
- Human PFKFB3 recombinant protein.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat PFKFB3
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R08-7A4
- Applications
- Western Blot, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 60 kDa; Observed MW: 60 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "PFKFB3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2204z | Mouse Anti-PFKFB3 Recombinant Antibody (clone 23H7) | ELISA, WB, IP | Mouse IgG2a, κ |
| MRO-1210-CN | Recombinant Rabbit Anti-PFKFB3 Monoclonal Antibody (JM43-43) | WB, IF, IHC, FC, IP | Rabbit IgG |
| VS3-WK587 | Rabbit Anti-PFKFB3 Recombinant Antibody (clone JM43-43) | WB, ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2670 | Hi-Affi™ Recombinant Rabbit Anti-PFKFB3 Monoclonal Antibody (DS2670AB) | WB, IP, ICC, IF, IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC872 | AbPlus™ Anti-PFKFB3 Magnetic Beads (VS-0724-YC872) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC896 | CytoStream™ Rabbit Anti-PFKFB3 Recombinant Antibody (VS13-YC896) | WB, ICC, IF, IHC-P, FC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5322 | Anti-PFKFB3 Immunohistochemistry Kit | IHC |
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