Recombinant Mouse Anti-POU5F1 Antibody (VS4-CJ81)

Name: Recombinant Mouse Anti-POU5F1 Antibody (VS4-CJ81)
SKU: VS4-CJ81
Rating: 5 (1 reviews)

CAT#: VS4-CJ81

This mouse anti-POU5F1 antibody is a product that recognizes human POU5F1. The immunogen of this antibody is recombinant full length protein (352aa) of mouse POU5F1. The mAb has been tested for use in ELISA, Western Blot (1:500-1:5000), Immunocytochemistry (1:50-1:200).

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line RT4

Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58267/1518_H6_1_selected.jpg

Subcellular Location and Protein Expression Figure 2 IHC staining of human hippocampus

Immunohistochemical staining of human hippocampus shows strong nuclear positivity in glial cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25600/ihc_selected.jpg

Normal Tissue Figure 3 Cerebral cortex

Glial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_B_8_5.jpg

Normal Tissue Figure 4 Colon

Endothelial cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_7_3.jpg

Normal Tissue Figure 5 Liver

Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_7_4.jpg

Normal Tissue Figure 6 Pancreas

Exocrine glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Pancreatic endocrine cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_3_3.jpg

Normal Tissue Figure 7 Kidney

Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_8_5.jpg

Normal Tissue Figure 8 Testis

Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25600/54340_A_6_6.jpg

Normal Tissue Figure 9 Lymph node

Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_8_8.jpg

RNA Expression Figure 10 RNA cell line category: Cell line enriched (SuSa)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000204531-POU5F1/subcellular

❮

❯

Specifications

Immunogen
Recombinant full length protein (352aa) of mouse POU5F1

Host Species
Mouse

Type
Mouse IgG1

Specificity
Human POU5F1

Species Reactivity
Human

Applications
ELISA, WB, ICC, FC

Conjugate
Unconjugated

Product Property

Purification
Affinity purified

Purity
>95% as determined by SDS-PAGE

Format
Liquid

Buffer
50% Glycerol, 0.1M Tris-Glycine (150 mM NaCl), pH 7.4.

Preservative
0.2% Sodium Azide

Storage
Store at -20°C or -80°C upon receipt. Avoid repeated freeze.

Applications

Application Notes
This antibody has been tested for use in ELISA, Western Blot (1:500-1:5000), Immunocytochemistry (1:50-1:200).

Target

Alternative Names
NF-A3; Oct-3; Oct-3/4; Oct-4; Oct3; Oct3/4; Oct4; Otf-3; Otf-4; Otf3; Otf3-rs7; Otf3g; Otf4

Gene ID
18999

UniProt ID
Q01860

Long Name
POU domain, class 5, transcription factor 1

Sequence Similarities
Belongs to the POU transcription factor family. Class-5 subfamily.

Cellular Localization
Cytoplasm, Nucleus

Post Translation Modifications
Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.
Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.
ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription.

Protein Refseq
NP_001272916.1; NP_002692.2

Function
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 or SOX15 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.

REVIEWS AND Q&AS CITATIONS RESOURCES DOWNLOADS RELATED PRODUCTS

Inquiry

Navs

Customer Review

There are currently no Customer reviews or questions for VS4-CJ81. Click the button above to contact us or submit your feedback about this product.

Submit Your Publication

Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS4-CJ81.pdf

MSDS

MSDS - VS4-CJ81.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Isotype Control

CAT
Product Name

Secondary Antibody

CAT
Product Name

See other products for "POU5F1"

Select a product category from the dropdown menu below to view related products.

CAT	Product Name	Application	Type
MOR-2797	Hi-Affi™ Recombinant Rabbit Anti-POU5F1 Monoclonal Antibody (DS2797AB)	FC, IHC-P, IP, WB	IgG
MOR-0065-FY	Rabbit Anti-POU5F1 Recombinant Antibody (clone AFY0036)	FC, ICC, IHC-P, WB	Rabbit IgG

CAT	Product Name	Application	Type
MRO-1126-CN	Recombinant Rabbit Anti-POU5F1 Monoclonal Antibody (CBACN-411)	WB, IF, IHC, IP, ChIP	Rabbit IgG
MRO-1127-CN	Recombinant Mouse Anti-POU5F1 Monoclonal Antibody (A3-C11)	WB, IF	Mouse IgG1
VS3-CJ41	Mouse Anti-POU5F1 Recombinant Antibody (VS3-CJ41)	WB, ELISA	Mouse IgG
VS3-XY1279	Mouse Anti-POU5F1 Recombinant Antibody (clone 1D10H6)	ELISA, WB	Mouse IgG1
VS11-QX120	Mouse Anti-POU5F1 Recombinant Antibody (clone 41B1)	IHC, WB	Mouse IgG2b, κ

CAT	Product Name	Application	Type
VS-0924-YC84	Mouse Anti-POU5F1 Recombinant Antibody (VS-0924-YC84) - Cancer Stem Cell Marker	IHC, WB	Mouse IgG1

CAT	Product Name	Application	Type
VS-0525-XY5587	Anti-POU5F1 Immunohistochemistry Kit	IHC
VS-0525-XY5588	Anti-Mouse POU5F1 Immunohistochemistry Kit	IHC

Specific Inquiry

Size:

Conjugation:

Endotoxin:

Purity:

Formats:

Isotype Switching:

Fc Engineering:

Modalities:

Add to compare

Request
Custom Production

See Our Custom Production in Action

Figure 1 Anti-HBV Recombinant Antibody (TAB-147) in SDS-PAGE

Download the full case study

Popular Products

Figure 5 GPNMB Recombinant Antibody (TAB-751) in ELISA

Human Anti-GPNMB Recombinant Antibody (clone Glembatumumab) (CAT# TAB-751)
Application: WB, IF, IP, Neut, FuncS, ELISA, FC

Figure 11 Blockade of PD-1/PD-L1 interaction by anti-PD-1 antibody (Cat# TAB-H55) resulted in an increase of IFN-γ production.

Human Anti-PDCD1 Recombinant Antibody (TAB-H55) (CAT# TAB-H55)
Application: Inhib, Agonist

Figure 2 Anti-CAIX/CA9 Antibody (TAB-707) in WB

Anti-CAIX/CA9 Recombinant Antibody (Girentuximab) (CAT# TAB-707)
Application: WB, FuncS, IF, Neut, ELISA, FC, IP

Figure 3 Anti-CD40 Antibody (TAB-126CL) in Dot Blot

Human Anti-CD40 Recombinant Antibody (TAB-126CL) (CAT# TAB-126CL)
Application: Block, Cyt, FuncS, Inhib

Figure 4 Human Anti-JAG1 Recombinant Antibody (PABL-312) in DB

Human Anti-JAG1 Recombinant Antibody (PABL-312) (CAT# PABL-312)
Application: IB, IF, IHC

Figure 1 Mouse Anti-α4β7 Recombinant Antibody (PABL-746) in ELISA

Mouse Anti-α4β7 Recombinant Antibody (PABL-746) (CAT# PABL-746)
Application: FC

Figure 6 Recombinant Human Anti-H3N2 HA Antibody (F045-092) (PABC-233) in DB

Recombinant Human Anti-H3N2 HA Antibody (F045-092) (CAT# PABC-233)
Application: Neut

Figure 2 Anti-Human CD47 Recombinant Antibody (TAB-303LC) in ELISA

Human Anti-CD47 Recombinant Antibody (TAB-303LC) (CAT# TAB-303LC)
Application: ELISA

Figure 4 Anti-Human CDCP1 Recombinant Antibody (TAB-403LC) in ELISA

Anti-Human CDCP1 Recombinant Antibody (25A11) (CAT# TAB-403LC)
Application: FC, FuncS, IHC

Figure 3 Anti-Human EFNB2 Recombinant Antibody scFv Fragment (B11) (TAB-390MZ-S(P)) in Dot Blot

Anti-Human EFNB2 Recombinant Antibody scFv Fragment (B11) (CAT# TAB-390MZ-S(P))
Application: ELISA

Figure 1 Anti-Human RNF43 Recombinant Antibody (TAB-327CT) in SDS-PAGE

Mouse Anti-RNF43 Recombinant Antibody (TAB-327CT) (CAT# TAB-327CT)
Application: WB, ELISA, FC, IHC, IP

Figure 5 Anti-Human RNF43 Antibody Fab Fragment (TAB-328CT-F(E)) in DB

Humanized Anti-RNF43 Recombinant Antibody; Fab Fragment (TAB-328CT-F(E)) (CAT# TAB-328CT-F(E))
Application: WB, ELISA, FC, IHC, IP

Figure 8 Anti-IAV HA Recombinant Antibody (MRO-276CT) in DB

Anti-IAV HA Recombinant Antibody (12D1) (CAT# MRO-276CT)
Application: ELISA, WB, Neut, Funcs, IHC, Inhib

Figure 2 Recombinant Mouse Anti-Streptococcus pneumoniae 23F Antibody (PABX-173) in HPLC

Recombinant Human Anti-Streptococcus pneumoniae 23F Antibody (023.102) (CAT# PABX-173)
Application: Neut, ELISA, FuncS

Figure 2 Recombinant Mouse Anti-HSPA1A Antibody (HPAB-0243-YC) in HPLC

Mouse Anti-HSPA1A Recombinant Antibody (HPAB-0243-YC) (CAT# HPAB-0243-YC)
Application: ELISA, FC

Figure 3 Mouse Anti-CEACAM6 Antibody (HPAB-0286-CN) in DB

Human Anti-CEACAM6 Recombinant Antibody (HPAB-0286-CN) (CAT# HPAB-0286-CN)
Application: ELISA, WB, FC

Figure 4 Recombinant Human Anti-SFTSV Antibody (MAb4-5) (HPAB-0032-WJ) in SEC-HPLC.

Recombinant Human Anti-SFTSV Antibody (MAb4-5) (CAT# HPAB-0032-WJ)
Application: Neut, ELISA, WB, SPR, IF, EM

Figure 14 Human Anti-IAV HA Antibody (PABC-393) in DB 4

Recombinant Human Anti-IAV HA Antibody (PABC-393) (CAT# PABC-393)
Application: Neut, FuncS, ELISA

Figure 2 Human Anti-Insulin Recombinant Antibody (clone mAb48) (FAMAB-0224WJ) in ELISA

Human Anti-Insulin Recombinant Antibody (clone mAb48) (CAT# FAMAB-0224WJ)
Application: ELISA, WB

Figure 4 Human Anti-Rotavirus VP8* Recombinant Antibody (clone mAb9) (PABJ-0170) in DB

Human Anti-Rotavirus VP8* Recombinant Antibody (clone mAb9) (CAT# PABJ-0170)
Application: ELISA, Neut

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.